lncRNA NEAT1在人脑胶质瘤的表达变化及其与病人预后的关系

目的 探讨长链非编码核糖核酸（lncRNA）NEAT1在人脑胶质瘤中的表达水平及其临床意义。方法 收集武汉大学人民医院神经外科2008年4月到2013年4月收治的65例脑胶质瘤标本及同期因颅脑手术切除的正常脑组织27例。65例胶质瘤中，WHO Ⅰ~Ⅱ级26例，Ⅲ~Ⅳ级39例。根据胶质瘤组织lncRNA NEAT1表达水平，将65例胶质瘤分成lncRNA NEAT1高表达组（n=38）和低表达组（n=27）。应用RT-PCR检测lncRNA NEAT1的表达水平，用Kaplan-Meier法进行生存分析，用多因素Cox比例风险回归模型分析lncRNA NEAT1的表达水平与病人预后关系。结果 胶质瘤组织lncRNA NEAT1的表达水平（2.893±0.296）显著高于正常人脑组织（1.000±0.297；P=0.001）。WHO Ⅰ~Ⅱ级胶质瘤lncRNA NEAT1的表达水平（2.160±0.270）显著低于WHO Ⅲ~Ⅳ级胶质瘤（3.627±0.312；P=0.005）。lncRNA NEAT1高表达组病人术后生存期明显缩短（P=0.033），术后5年累积生存率显著低于lncRNA NEAT1低表达组（P=0.033）。lncRNA NEAT1表达水平高、病理级别高、KPS评分<80分以及复发时间<3个月是影响胶质瘤病人术后预后的独立危险因素（P<0.05）。结论 lncRNA NEAT1在人脑胶质瘤组织中表达水平明显增高，其表达水平与胶质瘤病人预后显著相关。

Relationship of expression level of long non-coding RNA NEAT1 with prognoses in the patients with gliomas

Objective To study the expression level of the long non-coding RNA NEAT1 in glioma tissues and its clinical significance. Methods The levels of long on-coding RNA NEAT1 expression were determined by RT-PCR in 27 normal cerebral tissues samples and 65 gliomas tissues samples, of which, 26 were WHO grades Ⅰ~Ⅱ glioma samples and 39 WHO grades Ⅲ~Ⅳ. The survival rate in 65 patients with gliomas from whom the gliomas samples were derived was analyzed by Kaplan-Meier method and Log-rank. The factors related to the prognoses in these 65 patients with gliomas were univariate analysis and the Cox proportional risk regression model. Results The level of NEAT1 expression was significantly higher in the glioma tissues than that in the normal cerebral tissues (P<0.01). The level of the NEAT1 expression was significantly higher in the grades Ⅲ~Ⅳ gliomas tissues than that in the grades Ⅰ~Ⅱ gliomas tissues (P<0.01). The risk factors related to the prognoses in these 65 patients with gliomas included the high level of NEAT1 expression, high WHO grade, Karnofsky performance score<80 points and recurrence time <3 months. Conclusion The level of NEAT1 expression is significantly increased in the gliomas tissues compared to that in the normal cerebral tissues and is negatively correlated with the prognoses in the patients with gliomas. It is suggested that the level of NEAT1 expression in the gliomas tissues is an important index to predict the prognoses in the patients with gliomas.