Smac和survivin基因在骨肉瘤中的表达及其临床意义

目的探讨人骨肉瘤组织中Smac和survivin基因表达状态,及其对骨肉瘤生物学行为的影响。方法采用免疫组织化学技术(SABC法)检测46例骨肉瘤、10例骨软骨瘤组织中Smac和survivin蛋白表达,比较二者表达与骨肉瘤主要临床病理参数的关系。结果Smac和survivin基因在骨软骨瘤组织表达分别为3例、2例,骨肉瘤中分别为29例(63%)和31例(67%);Smac表达率与骨肉瘤组织学分级、WHO分型无关,与转移有关(P<0.05),Survivin表达率与骨肉瘤组织学分级无关,与WHO分型及转移有关(P<0.05);骨肉瘤中Smac和survivin基因表达呈正相关(r=0.506,P<0.01)。结论Smac和survivin基因高表达于骨肉瘤组织中,与骨肉瘤生物学行为密切相关,二者可能共同参与骨肉瘤的发生发展。

The expression and clinical significance of Smac and survivin gene in osteosarcoma

Objective To explore the expression of Samc and Survivin genes in osteosarcoma,and their effects on biological behaviour of osteosarcoma. Methods Using immunohistochemistry method (SABC) to detect the expression of Smac and Survivin protein in 46 osteosarcoma cases,10 osteochondroma cases,and then compare the expression of two genes with primarily clinical pathological parameter of osteosarcoma. Results The expression of Smac and Survivin gene was 3 cases(30%)and 2 cases (20%)in osteochondroma respectively,but in osteosarcoma was 29 cases (63%) and 31 cases(67%) respectively;the expression ratio of Smac has no relationship with osteosarcoma histology staging and WHO typing,but correlated with metastasis(P<0.05);the expression ratio of Survivin has no relationship with osteosarcoma histology staging,but correlated with WHO typing and metastasis(P<0.05);Smac has positive correlation with Survivin expression in osteosarcoma(r=0.506,P<0.01). Conclusion Smac and Survivin genes were both high expression in osteosarcoma,and correlated with biological behaviour of osteosarcoma closely;maybe they both involved in the occurrence and development of osteosarcoma.