Intramuscular nerve sprouting induced by CNTF is associated with increases in CGRP content in mouse motor nerve terminals |
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| Affiliation: | 1. Broad Institute of MIT and Harvard, Cambridge, MA, USA;2. Center for Computational and Integrative Biology and Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA;3. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA;4. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA;5. Boston University and NHLBI’s Framingham Heart Study, Framingham, MA, USA;6. Sections of Preventive Medicine and Epidemiology and Cardiology, Department of Medicine, Boston University School of Medicine, Boston, MA, USA;7. University of Texas School of Public Health, San Antonio, TX, USA;8. Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA;9. Harvard T.H. Chan School of Public Health, Boston, MA, USA;10. Division of Cardiovascular Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA;1. Department of Cardiovascular and Thoracic Surgery, The University of Louisville School of Medicine, Louisville, Kentucky;1. Research Center for Entrepreneurship and Family Firms (RCEF) at Hasselt University, Martelarenlaan 42, 3500 Hasselt, Belgium;2. Research Foundation Flanders, Egmontstraat 5, 1000 Brussels, Belgium;3. University of Antwerp, Prinsstraat 13, 2000 Antwerp, Belgium;1. Group of Biomedical Research in Digestive Tract Tumors, Vall d’Hebron University Hospital Research Institute (VHIR), Universitat Autònoma de Barcelona, 08035 Barcelona, Spain;2. Group of Molecular Oncology, Biomedical Research Institute of Lleida (IRBLleida), 25198 Lleida, Spain;3. Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007 Salamanca, Spain;4. Servicio de Transgénesis, Nucleus, Universidad de Salamanca, 37007 Salamanca, Spain;5. Departamento de Medicina, Universidad de Salamanca, 37007 Salamanca, Spain;6. Translational Molecular Pathology, Vall d''Hebron University Hospital Research Institute (VHIR), Universitat Autònoma de Barcelona, 08035 Barcelona, Spain;7. Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), 28029 Madrid, Spain;8. Digestive System Research Unit, Vall d’Hebron University Hospital Research Institute (VHIR), 08035 Barcelona, Spain;9. Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), Leuven 3000, Belgium;10. Group of Drug Delivery and Targeting, Vall d''Hebron University Hospital Research Institute (VHIR), Universitat Autònoma de Barcelona, 08035 Barcelona, Spain;11. Clinical Biochemistry Department, Vall d''Hebron University Hospital, 08035 Barcelona, Spain;12. Department of Biomedical Sciences of Cells and Systems, Section Molecular Cell Biology, University of Groningen, University Medical Center Groningen, Groningen 9713 GZ, the Netherlands;13. Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan;1. Universidad Nacional de Colombia, sede Medellín, A.A. 3840, Colombia;2. Universidad Nacional de Colombia, sede Manizales, Colombia |
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| Abstract: | It is known that motor nerve terminal sprouting induced by either nerve injury or muscle paralysis is associated with an increase in calcitonin gene-related peptide (CGRP) content in the soma of motoneurons and in motor endplates. In the present study, CGRP-like immunoreactivity (CGRP-LI) was determined in motor endplates of animals in which nerve terminal sprouting had been induced by exogenous application of ciliary neurotrophic factor (CNTF). After 18 days of CNTF treatment we observed a significant increase in CGRP-LI in motor endplates. The results indicate that CGRP is upregulated when motor nerve outgrowth is induced, even in the absence of muscle paralysis or nerve lesion. |
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