Cell intrinsic mechanisms regulate mouse cerebellar granule neuron differentiation |
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| Affiliation: | 1. School of Metallurgy & Environment, Central South University, Changsha, Hunan, 410083, China;2. Chinese National Engineering Research Center for Control & Treatment of Heavy Metal Pollution, Changsha, Hunan, 410083, China;3. Department of Green Chemistry, School of Engineering Science, LUT University, Sammonkatu 12, FI-50130, Mikkeli, Finland;4. Laboratory for Molecular Engineering of Optoelectronic Nanomaterials (LIMNO), École Polytechnique Fédérale de Lausanne (EPFL), Station 6, 1015, Lausanne, Switzerland;5. School of Civil Engineering and Surveying, Faculty of Health, Engineering and Sciences, University of Southern Queensland, Toowoomba, 4350 QLD, Australia;6. School of Chemical Engineering and Advanced Materials, The University of Adelaide, Adelaide, SA, 5005, Australia;1. Department of Earth and Oceanographic Science, Bowdoin College, Brunswick, ME, USA;2. School of Earth Sciences, Ohio State University, Columbus, OH, USA;3. Environment and Natural Resources Division, Abt Associates Inc., Bethesda, MD, USA;4. Department of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ, USA;5. Department of Earth and Planetary Sciences, Rutgers University, Piscataway, NJ, USA;1. School of Civil and Resources Engineering, University of Science and Technology Beijing, Beijing 100083, China;2. School of Automation and Electrical Engineering, University of Science and Technology Beijing, Beijing 100083, China |
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| Abstract: | Cerebellar granule cells isolated from postnatal day 7 mice, and cultured in minimal medium containing only insulin-like growth factor-I (IGF-I), both survive and differentiate. This differentiation is marked by neurite growth and expression of genes associated with terminal differentiation, the myocyte-specific enhancer factor 2A (MEF2A) and the α6 subunit of the γ-aminobutyric acidA receptor (GABAAα6). Percoll gradient purified granule cells maintained without IGF-I, in minimal medium alone or in medium containing the antioxidant N-acetylcysteine (NAC), also express MEF2A and GABAAα6. Thus, cultured granule neurons can differentiate to some extent cell-autonomously and IGF-I may not be a critical factor for this process. |
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