人乳头瘤病毒基因型检测在宫颈病变中的应用

目的研究HPV的基因亚型在不同程度的宫颈病变中的分布情况,以探讨不同基因型HPV感染的临床意义。方法采用HPV分型基因检测系统对妇科门诊就诊的1324例妇女进行宫颈细胞HPVDNA检测,对371例HPV感染的患者进行宫颈薄层液基细胞学检测(TCT)及阴道镜下宫颈多点活检,根据细胞和病理学诊断将其分为宫颈癌与高度鳞状上皮内瘤变(HSIL)组、低度鳞状上皮内瘤变(LSIL)组以及正常或炎症组,分析各组内HPV亚型的分布。结果HPV与宫颈癌变密切相关,随宫颈病变程度加重,高危型HPV基因型阳性率增加,且双重和多重感染有增加趋势;HPV-16、58、33、18、52和31在宫颈癌和HSIL组的感染率为59.8%、15.2%、12.2%、9.7%、9.1%和8.5%,说明这些亚型致癌型较强。结论HPV基因型的检测对预测宫颈病变进展、评估预后及指导治疗有重要意义。

The Applied Value of the Human Papilloma Virus DNA Checking for Cervical Diseases

Objective To investigate the human papillomavirus (HPV) types detected in patients with non-cancer, pre-cancer and cancer lesions at cervix and to analyze the oncogenesis of the various HPV types. Methods 1324 cases was tested by using the HPV Genotyping System, the cervical specimens from 371 patients with HPV infection were collected. All of the patients received Thin-layer liquidbased cytoiogy test(TCT) and colposcopy with multiple biopsy. Acorrding to the cytological and pathological diagnosis,the patients were divided into three groups such as cervical cancer and high-grade squamous intraepithelial lesions(HSIL) group, low- grade squamous intraepithelial lesions(LSIL) group, and normal or inflammatory group. The relationship between HPV types and different cervical diseases was analyzed. Results The positive rate of HPV-H rised as cervical diseases deepening, and there was a ascending trend of double or multiple HPV type infection. The infection rates of HPV types 16, 58, 33, 18, 52 and 31 in cervical cancer and HSIL were 59.8%, 15.2%, 12.2%, 9.7%, 9.1%and 8.5% respectively. It showed that HPV-H is closed associated with cervical cancer. Conclusion HPV-DNA genotyping is of great importance in diagnosis and prognosis of cervical disease.