HPLC法同时测定复合维生素口服液中4种水溶性维生素的含量

目的:建立测定复合维生素口服液中4种水溶性维生素的含量测定方法。方法:采用高效液相色谱法。同时测定复合维生素口服液中烟酰胺、维生素B6、维生素B1、维生素B2磷酸酯钠的含量,色谱柱为Diamonsil C18,流动相为己烷磺酸钠溶液-甲醇-冰醋酸(690∶303∶7,V/V/V),检测波长为280nm。结果:烟酰胺、维生素B6、维生素B1、维生素B2磷酸酯钠能实现良好的分离,检测浓度线性范围分别为87.5～700.0、7.50～60.0、25～200、17.5～140.0μg·mL-1(r均大于0.9993);平均回收率分别为101.3%、99.5%、100.0%和98.4%;RSD分别为0.5%、0.6%、0.6%和0.9%。结论:本方法简便、快速、准确且重复性好,为含有上述维生素类成分的多维复方制剂及保健产品的质量控制提供了参考。

Simultaneous Determination of 4 Kinds of Water-soluble Vitamins in Compound Vitamin Oral Solution by HPLC

OBJECTIVE： To establish a method for simultaneous determination of 4 kinds of water-soluble vitamins in Compound vitamin oral solution. METHODS： HPLC method was adopted. The content of 4 kinds of water-soluble vitamins, including nicotinamide, vitamin Br, vitamin BI and vitamin B2 sodium phosphate, were determined by HPLC. A Diamonsil Ct8 column was used. The mobile phase consisted of sodium hexanesulfonate solution-methanol-acetic acid （690 ： 303 ： 7, V/V/V） and the UV detection wavelength was 280 nm. RESULTS： 4 components were separated well and the calibration curves showed good linearity within the range of 87.5-700.0 μg.mL-1 for nicotinamide, 7.5--60.0 μg.mL-1 for vitamin Br, 25--200 μg.mL -1 for vitamin B1, and 17.5-140.0 gg.mL-1 for vitamin B2 sodium phosphate, respectively （r〉0.999 3）. The average recoveries were 101.3% （RSD= 0.5% ）, 99.5% （RSD=0.6%）, 100.0% （RSD=0.6%）, 98.4% （RSD=0.9%）, respectively. CONCLUSION： The proposed method is convenient, fast, accurate and reproducible. It provides reference for quality control of vitamins components of com- pound vitamin formulations and health product.