休克期切痂对烫伤大鼠血脂及血清游离脂肪酸谱的影响

目的　观察大鼠烫伤后不同时期切痂对血脂及血清游离脂肪酸 (FFA)的影响。　方法　 32只成年雄性大鼠制成 30 %TBSAⅢ度烫伤模型 ,分为单纯烫伤对照组、伤后 8、2 4、96h切痂治疗组 ,另设正常对照组 (8只 )。于伤后 16 8h处死大鼠 ,测定大鼠血脂及FFA谱的改变。　结果　 (1)大鼠伤后 16 8h ,血清甘油三酯 (TG)、胆固醇 (CHO)、高密度脂蛋白 (HDL)、低密度脂蛋白 (LDL)、极低密度脂蛋白 (VLDL)、载脂蛋白A (ApoA)、载脂蛋白B(ApoB)、血清总FFA中除肉豆蔻酸外各FFA水平均显著升高 (P <0 .0 5 ) ;(2 ) 8、2 4h切痂组的TG、CHO、ApoB水平、总FFA、月桂酸、软脂酸、软油酸、油酸、亚油酸水平显著低于同时相点烫伤对照组 (P <0 .0 5 ) ;8h切痂组VLDL、硬脂酸、花生四烯酸水平显著低于烫伤对照组 (P <0 .0 5 ) ;(3) 96h切痂组除ApoB、月桂酸水平显著低于同时相点烫伤对照组外 (P <0 .0 5 ) ,其他各指标均未降至伤前水平。　结论　严重烫伤后脂动员明显加强 ,休克期切痂植皮可降低脂动员 ,有利于正常脂代谢的恢复。

Effects of escharectomy during shock stage on the plasma lipid and serum free fatty acid levels in scalded rats

OBJECTIVE: To investigate the influence of escharectomy during shock stage on plasma lipid and free fatty acid levels in scalded rats. METHODS: Thirty-two adult Wistar rats inflicted with 30% TBSA III degree scalding were employed as the model and were divided into normal control (NC), scalding control (SC) and treatment groups (T), and the latter was further divided into three sub groups according to the time of escharectomy, i.e. 8 postburn hour (PBHs) (T8), 24 PBHs (T24) and 96 PBHs (T96) groups. The rats were sacrificed at 168 PBHs. The postburn changes in the rat plasma lipid and free fatty acid levels were determined. RESULTS: 1) There was significant increase in serum triglyceride (TG), cholesterol (CHO), high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), apolipoprotein A (ApoA), apolipoprotein B (ApoB) and all the free fatty acids (FFAs) in the total serum FFAs excluding myristic acid (P < 0.05) at 168 PBHs in rats of all the T groups. 2) The serum levels of TG, CHO, ApoB, total FFA, lauric acid, palmitic acid, zoomaric acid, oleic acid and linoleic acid in T8 and T24 groups were evidently lower than those in SC group (P < 0.05). The plasma levels of VLDL, stearic acid and arachidonic acid in T8 were obviously lower than those in SC group (P < 0.05); 3) In T96 group, the serum levels of ApoB and lauric acid were significantly lower than those in SC group (P < 0.05), but all the other indices remained higher than those before injury. CONCLUSION: There was enhanced fat mobilization after severe burn injury. Escharectomy during shock stage might decrease fat mobilization, which was beneficial to the restoration of normal lipid metabolism.