| 甲状腺乳头状癌与转移淋巴结组织蛋白质组学差异表达分析 |
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| 引用本文： | 边学.甲状腺乳头状癌与转移淋巴结组织蛋白质组学差异表达分析[J].中国耳鼻咽喉头颈外科,2011,18(7):363-366. |
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| 作者姓名： | 边学 |
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| 作者单位： | 北京军区总医院耳鼻咽喉头颈外科 |
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| 摘 要： | 目的 比较甲状腺乳头状癌(papillary thyroid cancer,PTC)与转移淋巴结(lymph node,LN)组织差异表达的蛋白质,筛选与PTC淋巴结转移的相关蛋白并初步探讨其转移机制.方法 利用固相PH梯度双相凝胶电泳技术,分离PTC以及LN组织的总蛋白,建立PTC与LN组织的蛋白表达谱.用Image...
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| 关 键 词： | 甲状腺肿瘤 蛋白质组学 电泳，凝胶，双向 光谱法，质量，基质辅助激光解吸电离 质谱分析法 淋巴结 |
| 收稿时间： | 2011-03-08 |
Analysis of different proteins expression between tissue of papillary thyroid carcinoma and cervical lymph node metastases |
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| BIAN Xue.Analysis of different proteins expression between tissue of papillary thyroid carcinoma and cervical lymph node metastases[J].Chinese Archives of Otolaryngology-Head and Neck Surgery,2011,18(7):363-366. |
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| Authors: | BIAN Xue |
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| Institution: | BIAN Xue(Department of Otolaryngology Head and Neck Surgery,Beijing Military General Hosptial,Beijing,100700,China) |
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| Abstract: | OBJECTIVE In order to understand the mechanisms of lymph node metastases and identify the different proteins expression in tissue of papillary thyroid cancer(PTC)and lymph node(LN).METHODS By using immobilized pH gradient(IPG)-based two-dimensional gel electrophoresis(2-DE),the total proteins were separated and 2-DE maps were established for tissue of PTC and lymph node(LN),respectively.The differential protein spots were analyzed with Image Master 2D Elite5.0 software,then the peptide mass fingerprinting o... |
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| Keywords: | Thyroid Neoplasms Proteomics Electrophoresis Gel Two-Dimensional Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry Lymph Nodes |
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