| 常年性持续性变应性鼻炎患者外周血单个核细胞CD45RA及CD45RO表达 |
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| 引用本文： | 罗鸿,余晔,沈莹,曾立刚,严能兵,柯峰,付选香,王鹏举,孔维佳.常年性持续性变应性鼻炎患者外周血单个核细胞CD45RA及CD45RO表达[J].中国耳鼻咽喉头颈外科,2011(5):260-262. |
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| 作者姓名： | 罗鸿 余晔 沈莹 曾立刚 严能兵 柯峰 付选香 王鹏举 孔维佳 |
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| 作者单位： | 襄阳市中心医院耳鼻咽喉科;襄樊学院医学院;华中科技大学同济医学院附属襄樊医院;华中科技大学同济医学院附属协和医院耳鼻咽喉科; |
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| 基金项目： | 科技部“十一五”科技支撑计划(2007BAI18B15) |
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| 摘 要： | 目的 探讨常年性持续性变应性鼻炎(AR)患者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中CD45RA及CD45RO的表达及其与AR发病的关系.方法 应用荧光抗体标记、流式细胞仪检测30例AR患者PBMC中CD45RA及CD45RO的表达.结果 与对照组比较,①AR...
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| 关 键 词： | 变态反应和免疫学 鼻炎 变应性 常年性 T淋巴细胞 调节性 T淋巴细胞亚群 流式细胞术 抗原 CD45 |
Expression of CD45RA and CD45RO in peripheral blood mononuclear cells of patients with allergic rhinitis |
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| LUO Hong,YU Ye,SHEN Ying,ZENG Ligang,YAN Nengbing,KE Feng,FU Xuanxiang,WANG Pengju,KONG Weijia.Expression of CD45RA and CD45RO in peripheral blood mononuclear cells of patients with allergic rhinitis[J].Chinese Archives of Otolaryngology-Head and Neck Surgery,2011(5):260-262. |
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| Authors: | LUO Hong YU Ye SHEN Ying ZENG Ligang YAN Nengbing KE Feng FU Xuanxiang WANG Pengju KONG Weijia |
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| Institution: | LUO Hong1,YU Ye1,SHEN Ying1,ZENG Ligang1,YAN Nengbing1,KE Feng1,FU Xuanxiang1,WANG Pengju1,KONG Weijia2(1 Department of Otolaryngology,the Central Hospital of Xiangyang City,Xiangfan Medical College,Affiliated Xiangfan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Xiangyang,Hubei,441021,China,2 Department of Otolaryngology,Union Hospital,Wuhan,430022,China) |
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| Abstract: | OBJECTIVE To investigate the expression of CD45RA and CD45RO in the peripheral blood mononuclear cells of patients with allergic rhinitis（AR）and their correlation with pathogenesis of allergic rhinitis.METHODS Double-label immunofluorescence procedures and flow cytometry were used for detecting the expression of CD45RA and CD45RO in peripheral blood mononuclear cells of 30 AR patients.RESULTS①CD3＋,CD3＋CD8＋ cells were decreased in AR patients compared with that in normal subjects,but there were no significant difference（P0.05）;② CD4＋ peripheral blood mononuclear cells in AR patients were significantly lower than that in normal control（P0.001）;③The ratio of CD3＋CD4＋/CD3＋CD8＋ in AR patients was lower than that in normal control（P0.001）;④CD45RA＋ cells in AR patients was significantly lower than that in normal control（P0.001） and CD45RO＋ cells in AR patients were significantly higher than that in normal control（P0.001）;⑤The ratio of CD45RO＋/CD45RA＋ in AR patients was higher than that in normal control（P0.001）;⑥ CD4＋CD45RA＋ cells in AR patients was significantly lower than that in normal control（P0.001）;⑦CD4＋CD45RO＋ cells and CD8＋CD45RO＋ cells in AR patients were higher than those in normal control（P0.001）;⑧The ratio of CD4＋CD45RO＋/CD4＋CD45RA＋ cells and CD8＋CD45RO＋/CD8＋CD45RA＋in AR patients were all significantly higher than that in normal controls（P0.01,P0.001）.CONCLUSION The results indicate that T-cell subsets in AR patients exist abnormalities,which results in the disturbance of body immune function and lead to AR exacerbation. |
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| Keywords: | Allergy and Immunology Rhinitis Allergic Perennial T-Lymphocytes Regulatory T-Lymphocytes Subsets Flow Cytometry Antigens CD45 |
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