LTA基因单核苷酸多态位点与胃癌易感性的研究

目的通过对江西地区汉族胃癌患者和正常人群中淋巴毒素-α（LTA）基因单核苷酸多态（single nucleotide polymorphism,SNP）位点rs909253基因型的检测,探讨rs909253位点与胃癌患者易感性的关系。方法利用Sequenom-MassArray-IPLEX检测194例胃癌和250例对照LTA基因多态位点rs909253的基因分型,并利用非条件logistic回归对检测结果进行相关统计。结果 LTA rs909253多态位点GG、GA、AA三种基因型在胃癌的频率分别为26.4%、52.0%和21.6%,与对照组（35.6%、46.4%和18.0%）相比,统计学上无显著意义（χ2=4.403,P=0.111）;但胃癌组和正常组的等位基因频率分布差异临近显著,P值达0.059;对年龄和性别进行校正后,携带等位基因G的胃癌发病风险有所增加（OR=1.315,95%CI：1.001~1.728）。结论江西汉族人群胃癌的遗传易感性可能与LTA基因rs909253位点的单核苷酸多态性有关,G等位基因为其发病的危险因素。

Investigation of single nucleotide polymorphism of LTA rs909253 with gastric cancer

Objective To investigate the association between a single nucleotide polymorphism （rs909253） of human lymphotoxinα（LTA） and gastric cancer susceptibility of Han Chinese population from Jiangxi province. Methods A case-control study was performed. 194 gastric cancers and 250 normal controls were examined, and Sequenome Iplex system and MALDI-TOF-MS were applied to detect the polymorphisms of rs909253 in LTA. We gathered statistics from test results with non-conditional logistic model. Results The frequencies of genotypes GG, GA and AA at SNP rs909253 were 26.4%, 52.0% and 21.6% in gastric cancer respectively, which were not significantly different from that in controls （35.6%, 46.4% and 18.0%; χ 2 =4.403, P=0.111）. But there was a difference on rs909253 allele frequency between gastric cancer and healthy people, near statistical significance（χ 2 =3.576, P=0.059）. The relative risk in the carrier of G allelic gene was higher than that in the carriers of A （OR=1.315, 95% CI： 1.001~1.728）. Conclusion The single nucleotide polymorphisms of LTA rs909253 G allele are possible related to genetic susceptibility of gastric cancer.