NBQX对头部照射大鼠大脑皮质少突胶质前体细胞的保护作用

目的　探讨大鼠头部照射后早期大脑皮质少突胶质前体细胞的放射性反应及MK80 1和NBQX对其保护作用。方法　SD大鼠 10Gy单次头部照射后即分别向其脑皮质立体定向注射 5mmol L的MK80 1和 5 0mmol L的NBQX各 1μl,然后用免疫荧光组织化学法分别检测各时间点大脑皮质NG2和O4阳性细胞数量。结果　和对照组相比 ,照射组大鼠照射后 7d时大脑皮质NG2和O4阳性细胞数显著增加 (P <0 0 5 ) ;MK80 1组大鼠照射后各时间点大脑皮质NG2和O4阳性细胞数与照射组大鼠比较差异无统计学意义 (P >0 0 5 ) ;而NBQX组大鼠照射后 1和 7d时的大脑皮质NG2和O4阳性细胞数明显多于照射组大鼠 (P <0 .0 5 )。结论　大鼠头部照射后早期大脑皮质少突胶质前体细胞反应性增多 ,并有时程性变化 ;NBQX对少突胶质前体细胞早期的放射性损伤有一定的保护作用。

The neuroprotective effect of NBQX on oligodendrocyte precursor cells in rat cerebral cortex after whole brain irradiation

Objective To explore the early phase radioresponse of oligodendrocyte precursor cells in ratcerebral cortex after whole brain irradiation and the protective effect of MK801 and NBQX. Methods MK801 (5 mmol/L) or NBQX(50 mmol/L) was injected into the cerebral cortex of SD rats by using a stereo-directional instrument after whole brain irradiation at dose of 10 Gy.Then NG2 and O4-positive cells in the cortex were determined at each time-point by immunofluorescence histochemistry. Results The numbers of NG2 and O4-positive cells in the cortex of rats in the irradiation group increased significantly on day 7 after irradiation as compared with those in the sham irradiation group (P<0.05).There was no obvious difference for the number of NG2 or O4-positive cells in the cortex between rats of the MK801 group and the irradiation group (P>0.05).The numbers of NG2 and O4-positive cells in the cortex of rats in the NBQX group were significanctly higher on days 1 and 7 than that those of rats in the irradiation group (P<0.05). Conclusion The number of oligodendrocyte precursor cells in the cortex of rats increases reactively in the early phase after whole brain irradiation and has alteration with observing time-point.And NBQX has some neuroprotective effect on oligodendrocyte precursor cells in the early phase of radiation injury.;