胃癌细胞多药耐药基因相关蛋白和p53表达及其相互关系

目的：研究胃癌细胞中多药耐药基因相关蛋白（multidrug resistance-associated protein,MRP)和抑癌基因p53的表达以及与胃癌生物学行为之间的关系。方法：用免疫组织化学方法对40例胃癌组织标本MRP及p53表达进行检测。结果：MRP阳性率为45％（18／40）。MRP阳性病例中p53阳性率为44．4％（8／18）。MRP阴性病例中p53阳性率为40．8％。MRP的表达与胃癌大体类型、组织类型、浸润深度、淋巴转移、远隔转移等因素无关。与p53的表达之间关系无显著意义。结论：MRP在胃癌组织中有较高水平的表达，其表达水平与胃癌生物学行为特点无关。p53对MRP的表达无调控作用。

Expression and Clinical Relavance of Multidrug Resistance-associated Protein and p53 in Human Gastric Cancer

Objective: Our aim was to examine the relationship between the expression of multidrug resistance associated protein (MRP) and p53, also,and the biologic factors regarding invasion and metastasis of human gastric cancer. Methods: In 40 patients with gastric cancer, the expression of MRP was immunohistochemically investigated. The relationship between MRP expression and development, invasion, and metastasis of gastric cancer was analyzed. The overexpression of the tumor suppressor gene p53 was investigated immunohistochemically. Results: Immunohistochemically, MRP positive was noted in 18 of 40 tumors(45%). Positive p53 staining was evident in 8 of 18 MRP positive tumors (44.4%) and 9 of 22 negative ones(40.8%). There was no significant correlation between anatomical types, histological types, invasion, metastasis of gastric cancer and MRP abnormal expression. Conclusion: There was no significant correlation between MRP and p53 staining. The expression of MRP is independent of known factors related to the development, invasion, and metastasis of human gastric cancers.