调气中药对豚鼠体外胃平滑肌运动的影响

[目的]观察同归脾、胃经具有调理气机功能的莱菔子、大腹皮、白术、小茴香、莪术,香附、蒲公英等7味中药对豚鼠体外胃纵行肌条的收缩作用,探讨其促胃动力作用规律。[方法]制备豚鼠胃底、胃体平滑肌条,以0.85%氯化钠(NS)和乙酰胆碱(ACh)作对照,观察7味中药对豚鼠体外胃纵行平滑肌的收缩效应。[结果]7味中药对胃底胃体纵行肌条均有不同程度的兴奋作用。与NS组比较,差异有统计学意义(P<0.01)。以ACh标化实验数据,莱菔子的收缩作用最强,与大腹皮比较,差异无统计学意义(P>0.05),与其他5味中药比较,差异有统计学意义(P<0.05),白术、蒲公英、小茴香的作用次之,无论在胃体还是胃底,莪术和香附的收缩作用均稍弱,分别与其他5味药比较差异均有统计学意义(P<0.05)。[结论]7味中药均有较强的促胃动力作用,其中莱菔子、大腹皮作用显著,值得进一步开发研究。从脾胃论治,结合气机升降理论开展促胃肠动力中药筛选是一条有效途径。

Study of traditional Chinese Qi-regulating medicine on contractile activity of guinea pig gastric smooth muscle in vitro

[Objective]To study the effect of raphani,areca peel,atractylodes,cumin,taraxaci,curcumae zedoaria,cyperus rotundus on contractile of isolated guinea pig gastric longitudinalmuscle strips and investigate the effect of different Chinese herbal medicine belonging to speen-stomach channel.[Methods]Longitudinal muscle strips of guinea pig corpus gastricum and fundus gastricus were prepared and 0.85% saline and acetylcholine were used as control.The contraction effect of traditional Chinese medicine on gastric smooth muscle strip was observed.[Results] The seven Chinese herbal medicine can induce contraction of smooth muscle strips in different degrees compared with the saline group(P<0.01).The result of experiment was standardized by ACh.Contraction effect of raphani was more powerful than the other herbal groups in fundus gastricus as well as in corpus gastricum(P<0.01).The effect of nutgrass and zedoary was weaker than the other groups(P<0.05).[Conclusion ]In the promotion of stomach smooth muscle strips contraction aspect,the seven kinds of Chinese herbal medicine has strongly kinetogenic gastrointestinal effect.The effect of areca peel and raphan are more significant.It is a way to screen the kinetogenic gastrointestinal Chinese herbal medicine based on the theory of spleen-stomach and the functional activity of Qi.