系统性红斑狼疮患者外周血单一核细胞白介素6受体水平的研究

目的 探讨白介素6(IL-6)及其受体(IL-6R)在系统性红斑狼疮(SLE)发病中的作用。方法 应用逆转录酶-聚合酶链反应(RT-PCR)检测89例SLE患者和30例正常人外周血单一核细胞(PBMC)中IL-6RmRNA的表达水平,用ELISA法检测血清中IL-6水平。结果 ①活动期SLE患者和非活动期SLE患者及正常人IL-6R阳性表达率为100%。②PBMC中,IL-6RmRNA表达水平:活动期SLE患者为0.902±0.273,非活动期SLE患者为0.519±0.11,正常人为0.573±0.24。活动期组和非活动期组和对照组比较,差异均有统计学意义(P=0.00),非活动期组和对照组比较差异无统计学意义(P=0.289)。③血清中IL-6水平:活动期组为71.89±20.02pg/mL,非活动期组为17.96±6.93pg/mL,对照组为0.035±0.035pg/mL。活动组显著高于非活动组和对照组(P=0.00),非活动组显著高于对照组(P=0.00)。活动期SLE患者和非活动期SLE患者血清中的IL-6水平与PBMC上的IL-6R的表达水平呈正相关(r=0.887和r=0.615,P<0.05)。结论 IL-6及其受体的异常表达可能在SLE疾病活动和发展过程中起重要作用。检测SLE患者PBMC中IL-6受体的表达水平可作为评估疾病活动性的参考指标之一。

IL-6 Receptor Expression in Peripheral Blood Mononuclear Cells of the Patients with Lupus Erythematosus and its Clinical Significance

Objective To investigate the role of IL-6 and IL-6R in the pathogenesis of systemic lupus erythematosus (SLE) and its clinical significance. Methods RT-PCR technique was used to semiquantitatively analyze IL-6R mRNA expression in peripheral blood mononuclear cells (PBMC), and double antibody sandwich ELISA was applied to determine the serum level of IL-6 from 89 SLE patients. Results The positive IL-6R mRNA expression was found in each individuals among active SLE group (groupⅠ), inactive SLE group (groupⅡ) and control group (group Ⅲ). The mean levels of IL-6R mRNA expressions in group Ⅰ,Ⅱ or Ⅲ were 0.902±0.273, 0.519±0.11 and 0.573±0.24, respectively. Compared with group Ⅱ and group Ⅲ, the mean level of IL-6R mRNA expression in group Ⅰ was markedly increased (P=0.00), while there was no statistical difference between group Ⅱ and group Ⅲ(P=0.289). The mean serum levels of IL-6 in group Ⅰ,Ⅱ and Ⅲ were 71.89±20.02,17.96±6.93, and 0.035±0.035, respectively. Compared with group Ⅱ and group Ⅲ, the mean level of IL-6 in groupⅠ was significantly higher than those in group Ⅱ and group Ⅲ (P=0.00), while that in group Ⅱ was higher than group Ⅲ (P=0.00). There was a positive correlation between IL-6 and IL-6R in active SLE group and inactive SLE group(r=0.887, and r=0.615 P<0.05). Conclusions The expression of IL-6R mRNA in PBMC and the serum level of IL-6 in active SLE patients are significantly higher than those in inactive SLE patients and control group. The serum level of IL-6 in inactive SLE patients is higher than that in control group. The abnormal expression of IL-6 and IL-6R might play an important role in the pathogenesis of SLE, and the level of IL-6R might be used as one of the referential indicators for active SLE.