肿瘤坏死因子α基因多态性与多囊卵巢综合征发病的关系

目的 探讨肿瘤坏死因子α（ＴＮＦ－α）基因多态性与多卵巢综合征（ＰＣＯＳ）发病的关系。方法 用聚合酶链反应、限制性内切酶消化及电泳技术，对１１８例ＰＣＯＳ患者和５４例对照者ＴＮＦ－α基因启动子区－３０８位碱基多态性进行检测。出现８７／８７片段定义为ＴＮＦ１基因型，出现８７／１０７和１０７／１０７片段定义为ＴＮＦ２基因型。比较两种基因型在ＰＣＯＳ患者和对照者的分布及其与ＰＣＯＳ患者糖代谢特征的关系。

Study on the relationship between a polymorphism of tumor necrosis factor-alpha gene and the pathogenesis of polycystic ovary syndrome]

OBJECTIVE: To investigate the relationship between -308 bp polymorphism in tumor necrosis factor-alpha (TNF-alpha) gene promoter region and the pathogenesis of polycystic ovary syndrome (PCOS). METHODS: One hundred eighteen Chinese women with PCOS (44 were obese, and 74 nonobese) and 54 controls were genotyped according to the fragment length [87 bp and(or) 107 bp] of TNF-alpha gene promoter by the technique of polymerase chain reaction-restriction fragment length polymorphism. The distribution of TNF1 genotype (87/87) and TNF2 genotype (87/107 + 107/107) in the two groups and their relations to parameters of glucose metabolism associated with PCOS were compared. RESULTS: There was no significant difference in the distribution of TNF1 and TNF2 genotypes between PCOS group and the controls (P > 0.05). In obese PCOS group, only significant lower insulin sensitivity index was found in TNF-1 genotype as compared with TNF2 genotype (P < 0.05). In nonobese PCOS group, there was no significant difference in all parameters of glucose metabolism between TNF1 and TNF2 genotype. All parameters of glucose metabolism in obese patients with TNF1 genotype were significantly different from those in nonobese with TNF1 genotype. However, significant differences were found only in some parameters when comparison was made between obese and nonobese within the TNF2 genotype group. CONCLUSION: There is no association between TNF-alpha gene -308 bp polymorphism and the pathogenesis of polycystic ovary syndrome, but PCOS patients with TNF2 genotype may associate with improvement to certain degree on abnormal glucose tolerance usually existed in obese PCOS women.