急性运动对小鼠心肌线粒体功能的影响

目的：探讨不同强度运动对心肌线粒体功能的影响。方法：40只小鼠随机分为安静对照组（A组）、90min运动组（B组）、力竭组（C组）及力竭后恢复24h组（D组）各10只，以游泳为运动方式，观察各组血清肌酸激酶（CK）、乳酸脱氢酶（LDH）、ATP合成活性及脂质过氧化水平丙二醛（MDA）的变化，并在光镜和电镜下观察心肌超微结构。结果：与A组比较，C组血清CK、LDH水平及心肌MDA水平显著增高（P〈0．05）．ATP合成能力显著下降（P〈0．05），电镜下心肌超微结构损伤明显。结论：急性力竭运动可造成线粒体损伤和心肌损伤，力竭运动后24h各项指标基本恢复。

Effects of Exercises on Function of Myocardial Mitochondria in Mice

Objective:To study the changes of mitochondrial function in myocardiocytes after exercise in mice.Methods:Forty mice were randomly divided into four groups: control,90-min swimming,exhaustive swimming and 24-h recovery after exhaustive swimming.The changes in the serum creatine phosphokinase(CK) activities and lactate dehydrogenase(LDH),myocardial malondialdehyde(MDA) and ATP activities were investigated and myocardial tissue structures were observed.Results :As compared with the control group,the serum CK and LDH were higher(P<0.05),the myocardial MDA was higher(P<0.05),the ATP activities were lower(P<0.05) in exhaustive swimming group.Electron microscopy revealed that myocardial structures in exhaustive swimming group were injured.Conclusion: Exhaustive exercise could result in injures of mitochondria and myocardiocytes.Twenty-four h after exhaustive exercise,various indexes were basically recovered.