天麻素对缺血再灌注损伤星形胶质细胞的保护作用及其对一氧化氮合成酶活性的影响

观察天麻素对脑缺血再灌注损伤星形胶质细胞胶原纤维酸性蛋白 (GFAP)表达、乳酸脱氢酶(LDH)漏出量以及一氧化氮合成酶 (NOS)活性的影响。结果 :天麻素大、小剂量组GFAP纤维样改变减轻 ,强阳性反应细胞数减少 ;LDH漏出量下降 ;NOS活性减弱。提示 :天麻素对模拟脑缺血再灌注损伤星形胶质细胞有良好的保护作用 ,其途径之一可能是通过抑制NOS活性的反应性增强来实现的。

Protective Effects of Gastrodine Against Lesions in Cultured Astrocytes Caused by Simulated Cerebral Ischemia and Reperfusion, and Its Influence on the Activity of Nitric Oxide Synthase

The effects of gastrodine on the expression of glial fibrillary acidic protein (GFAP), the transuding amount of lactic dehydrogenase (LDH), and the activity of nitric oxide synthase (NOS) in cultured astrocytes of young rats with lesions caused by simulated cerebral ischemia and reperfusion, were determined in this experiment. The results showed that in both the large dose gastrodine group and small dose gastrodine group, GFAP fibroid changes were reduced with less astrocytes being in an intense positve reaction, LDH transuding amount was lessened and NOS activity was decreased. The results suggest that gastrodine has very good protective effects against lesions caused by simulated cerebral ischemia and reperfusion in cultured astrocytes, and its inhibiting the increased activity of NOS may be one of its mechanisms of effect.