胃癌组织学多参数诊断价值评估

目的本文为实施临床胃癌诊断研究,对组织学多参数综合诊断分析进行了详估。方法运用 ABC 免疫组化技术检测 MG—3c7抗原和 cerbB-2受体在胃癌组织中的表达,同时用流式细胞术(FCM)测定组织标本中 DNA 含量。方法学多参数判断值经统计学分析处理。结果 MG—3c7抗原与 cerbB-2受体在胃管状腺癌中的表达率分别为100%和56%,腺癌为58%和50,胃正常粘膜为20%和33%。DNA 异信体率在胃管状腺癌中占63%(12/19),腺癌占75%(3/4),正常粘膜占0%(0/8)。ABC 与 FCM 检测胃管状腺癌的方法学参数综合分析表明,敏感性为82.3%,特异性为88%,预期值为95.4%,准确性为84%,证明两种实验诊断方法具有一定的互补性。结论多参数综合诊断分析和组织形态学及分子生物学研究为胃癌的组织学诊断提供了一条新的途经。

Evaluation of multi- parameter diagnosis in histology of gastric cancer

AIMS The synthetic diagnosis and analysis of multiparameter in histology is evaluated for the clinical study on diagnosis of gastric cancer(GC). METHODS The expressions of MG-3c7 anatigen and cerbB-2 receptor in specimens of GC were detected with the technique of ABC immunnohistochemistry.The DNA content of specimens was determined with flow cytometry(FCM).The data of multi-parameter in methodology were analysed by statistics. RESULTS The expressive rates of MG-3c7 antigen and cerbB-2 receptor were observed in gastric tublar adenocarci- noma(GTA,100% and 56%),adenocarcinoma(AC,58% and 50%),and normal gastric mucosa(NGM,20% and 33%). respectively The DNA heteroploid rate was 63%(12/19)in GTA,75%(3/4)in AC and 0%(0/8)in NGM.In Detecting GTA with FCM and ABC method,the histological parameter analysis synthetically showed that the sensitivity was 82.3% specificity 88%,expentancy 95.4% and accuracy 84%.Both methods were complementary. CONCLUSIONS The synthetic diagnosis and analysis of multi-parameter and the study on histomorphology and molecular biology may be a new way for the diagnosis on GC in histology.