子宫肌瘤中细胞凋亡受体mRNA表达水平分析

目的研究子宫肌瘤与细胞凋亡受体表达的关系，探讨子宫肌瘤的发生机制。方法采用半定量RT-PCR方法对20份子宫肌瘤样本进行了细胞凋亡受体mRNA表达水平的检测。结果子宫肌瘤中DcR1、DcR2、DR5、TNFR1A、TNFR1B、Fas等mRNA的表达水平与正常组织差异无统计学意义（P〉0．05）；然而肿瘤坏死因子细胞凋亡受体DR4在正常组织中mRNA的表达水平为（1．01±0．18），比子宫肌瘤组织样本的（0．58±0．27）显著降低（P〈0．001）。结论子宫肌瘤的发生很可能与DR4表达降低有关，其确切的分子机制有待深入研究。

Decreased mRNA expression of death receptor(DR)-4 in the leiomyoma tissues

Objective To investigate the association of leiomyoma with death receptors(DR) and the mechanisms underlying the development of leiomyoma.Methods The semi-quantitative RT-PCR was employed to examine the mRNA expression of apoptosis-associated death receptors in the Leiomyoma tissue samples collected from 20 clinical cases.Results It was found that the mRNA expression of DR4 in leiomyoma tissue was dramatically decreased as compared to that of normal tissue controls (P<0.001),but no difference was found between leiomyoma and normal tissue controls in terms of DcR1,DcR2,DR5,TNFR1A,TNFR1B and Fas mRNA expressions (P>0.05).Conclusion It is highly possible that DR4 expression might be involved in the development of leiomyoma.More efforts will be required to elucidate the exact mechanisms underlying the development of leiomyoma.