针刺对兔脑损伤后氧自由基反应的影响

目的探讨针刺治疗对急性兔颅脑损伤(ACCI)后脑组织氧自由基反应的影响。方法48只家兔随机分为A组(正常对照组)、B组(假损伤组)、C组(损伤组)、D组(针刺治疗组),Feeney’s自由落体打击法建立ACCI动物模型,D组给予针刺治疗,检测A、B组脑组织含水量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和C、D组不同时间点上述指标的变化。结果A与B组各检测指标差异无统计学意义(P>0.05);与A、B组比较,C、D组脑组织含水量和MDA含量在ACCI后3h即有显著性增加(P<0.01),且随时间延长递增,SOD活性则呈相反变化趋势;D组与C组各时点比较,上述指标变化均较轻。结论兔ACCI后脑组织自由基反应增强,针刺治疗可通过降低脑组织氧自由基反应从而减轻脑水肿。

Influence of Acupuncture on Oxygen Free Radical Reaction in Rabbit Brain Tissues After Craniocerebral Injury

Objective To investigate the influence of acupuncture on oxygen free radical reacton in rabbit brain tissues after acute craniocerebral injury (ACCI). Methods 48 rabbits were randomly divided into group A (normal control group ) , group B ( sham injured group) , group C (injured group) and group D (acupuncture treatment group ) . The model of ACCI was performed by free dropping weight . Animals in group D was treated by acupuncture after ACCI. Then the contents of brain water , malonyl-diadehyde (MDA) and activity of superoxide dismutase (SOD) were measured. Results The indexes measured had no statistic difference between group A and B(P>0.05) ; Compared with group A and B , contents of brain water and MDA were significantly increased at 3 hours after ACCI (P<0.01) , and kept on ascending with time extended. SOD activity was remarkably decreased ; Compared with group C , the changes of above indexes of group D were slighter. Conclusions Oxygen free radical reaction in rabbit brain tissues was enhanced after ACCI. Acupuncture can alleviate brain edema by attenuating free radical reaction.