脑缺血再灌流脑微血管损害及u-PA表达的实验研究 |
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引用本文: | 李玲,黄如训,吴金浪,陈燕奎,苏镇培. 脑缺血再灌流脑微血管损害及u-PA表达的实验研究[J]. 中国神经精神疾病杂志, 1998, 0(5) |
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作者姓名: | 李玲 黄如训 吴金浪 陈燕奎 苏镇培 |
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作者单位: | 中山医科大学附属第一医院神经科 |
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摘 要: | 目的探讨脑缺血再灌流后继发的脑水肿、出血的发生机制。方法应用光镜、透射电镜、免疫组织化学、显微镜-计算机图像分析等技术,观察大鼠局部脑缺血2小时再灌流不同时间,脑微血管结构、Ⅳ型胶原抗原及尿激酶型纤溶酶原激活物(u-PA)表达。结果局部脑缺血再灌流24小时,缺血侧MCA区脑微血管外细胞间质水肿最严重,基底膜节段性溶解、缺损,有红细胞漏出,微血管壁及管外细胞间质u-PA大量表达达高峰,同时微血管基底膜Ⅳ型胶原抗原减少。随再灌流时间延长,微血管基底膜损害加重,Ⅳ型胶原抗原逐渐消失,u-PA表达减少。结论脑缺血再灌流后脑微血管结构损害是导致脑水肿、出血的主要病理基础,而脑微血管壁和管外细胞间质u-PA表达可能是引起微血管损害的主要机制之一。
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关 键 词: | 局部脑缺血 再灌流 尿激酶型纤溶酶原激活物 脑微血管结构 |
The experimental study on cerebral microvascular and u-PA after local cerebral ischemia with reperfusion. |
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Abstract: | Objective To investigate the mechanism of brain edema and hemorrhage after focal cerebral ischemia with reperfusion. Methods The cerebral microvascular ultrastructure and the product of u-PA in microvascular and intercellular substance after focal cerebral ischemia with reperfusion were observed by transmission electron microscope, immunohistochemical method and morphological analysis. Results The results showed that u-PA on cerebral ischemic area was significantly increased after focal cerebral ischemia with reperfusion 6 hours. By 12-hour reperfusion, cerebral microvascular structure was damaged and type IV collagen of microvascular reduced at the ischemic area. Conclusions These results suggest that u-PA might play an important role in microvascular lesion after reperfusion following cerebral ischemia. |
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Keywords: | Focal cerebral ischemia Reperfusion u-PA Cerebral microvascular structure |
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