自制双试剂测定血清胆红素的探讨

目的；建立准确的自动生化分析测定血清胆红素的方法。方法：采用双试剂测定，以尿素咖啡因及Ｔｒｉｔｏｎ－Ｘ１００组成的加速剂作为第一试剂，重氮试剂为第二试剂测定总胆红素，以对氨基苯碘酸溶液为第一试剂，亚硝酸钠浓度为１．４５ｍｍｏｌ／Ｌ的重氮试剂为第二试剂测定直接胆红素。结果：总胆红素回收率为９９．８５％，批内艰０．８２％，批间ＣＶ为１．５４％，与咖啡因法比较ｒ＝０．９９９９。ｔ＝１．３２３，Ｐ〉０．２

Preparation of double-reagent for determining the serum bilirubin

objertive To establish accurate automated analysis procedure for determining the serum bilirubinMethods Urea .caffeine and Trion - X 100 were used as the first reagent and diazo p - amino - benxene -sulphonic acid anhy as the second reagent for determining the total bilirubin. To measure the direct bilirubin, the p -aniobenxene -sulphonic acid anhy was used as the first reagent and 1. 45 mmol/L sodium nitrite diazo - p-aminobenxene-sulphonic acid anhy as the second reagent. Results The total bilirubin concentrations measured by this procedure. (Y) in 90 sera correlated well with those results obtained with the modify Jendrassik-Grof(X), Y=1. 144X- 3.83,r= 0. 999 9. Within-run was 0. 82% and between -run was 1. 54% respectively. The calibration curves was linear in range of 0 to 513 umol/L. The recovery rate was between 99. 7 and 101. 0%. The direct bilirubin results was correlated well with the modify Doumas' (x ) Y= 0. 982X 0. 25,r=0.998 6 (n= 90). Within -run was 1. 20% and between-run was 1. 91 % respectively. The common interference were not effected, but bemoglobn pede a reduce effect. The reagents were stable for three months. Conclusions the results determinate by this procedure were accurate and the reagents were stable. It may be used for routine method.