| 睾丸电镜酶组织化学标本制备方法研究 |
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| 引用本文： | 李玉绿,文建国,周军,卢迪生. 睾丸电镜酶组织化学标本制备方法研究[J]. 中南大学学报(医学版), 1994, 0(6) |
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| 作者姓名： | 李玉绿 文建国 周军 卢迪生 |
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| 作者单位： | Department of Histology and Embryology，Hunan Medical University |
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| 摘 要： | 本文对曲细精管薄片标本的制备作了较大改进，技术要点为原位灌注固定加振动切片。改进后曲细精管入水不松散，即使振动孵育，组织片亦保持完整，超微结构完好清晰，酶反应产物定位良好。表明本法适用于睾丸电镜酶组织化学研究。
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| 关 键 词： | 组织细胞化学，酶，显微镜检查，电子，睾丸，方法 |
THE IMPROVEMENT OF TESTIS SPECTMEN PKEPARATION WITH ENZYMATICHISTOCHEMICAL TECHNIQUE IN ELECTRON MICROSCOPY |
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| Li Yulu,Wen Jianguo,Zhou Jun,Lu Disheng. THE IMPROVEMENT OF TESTIS SPECTMEN PKEPARATION WITH ENZYMATICHISTOCHEMICAL TECHNIQUE IN ELECTRON MICROSCOPY[J]. Journal of Central South University. Medical sciences, 1994, 0(6) |
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| Authors: | Li Yulu Wen Jianguo Zhou Jun Lu Disheng |
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| Abstract: | eminiferous tubules scattered after being immersed in water,This was always anunresolved problem in research of EMenzymatic histochemistry of testis.An obviousimprovement for specimen preparation was reported in this paper.The main techniques wereperfusion fixation in situ and vibration sections.By using the new method,the seminiferoustubules no longer scattered in water,the tissue pieces were well preserved even incubated invibration condition,The ultrastructure was clear and well preserved.The reaction products ofenzymes was precisely located.It is suggested that this method is suitable for research onenzymatic histochemical technique of testis in electron microscopy. |
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| Keywords: | histocvtochemistry enzymes microscopy electron testis methods |
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