| 大肠侧向发育型肿瘤中sfrp1基因存在高甲基化 |
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| 引用本文： | 翟国栋,王新颖,龚伟,姜泊. 大肠侧向发育型肿瘤中sfrp1基因存在高甲基化[J]. 现代消化及介入诊疗, 2011, 0(2): 105-108 |
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| 作者姓名： | 翟国栋 王新颖 龚伟 姜泊 |
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| 作者单位： | 南方医科大学南方医院消化病研究所; |
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| 摘 要： | 目的研究Wnt信号途径的抑制基因（sfrp1、sfrp2、sfrp5）在侧向发育型肿瘤（LST）及隆起型腺瘤（PA）中的表达情况及甲基化状态。方法运用半定量PCR法和免疫组织化学方法研究52例LSTs和51例PAs组织标本sfrp1、sfrp2、sfrp5表达情况;运用甲基化特异性PCR法（MSP）对3个抑制基因甲基化状态进行研究。结果 LSTs组sfrp1mRNA及蛋白表达水平均比PAs组显著降低（P〈0.05）,但sfrp2及sfrp5mRNA表达在LSTs组和PAs组中无显著差异（P〉0.05）。LSTs组中的sfrp1基因存在高甲基化（P〈0.05）,sfrp2及sfrp5基因不存在高甲基化（P〉0.05）。结论 sfrp1表达缺失或下调在LSTs比PAs更常见,该基因高甲基化发生频率在LSTs也相应比PAs高,提示LSTs的发病机制可能与PAs存在不同。
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| 关 键 词： | 大肠侧向发育型肿瘤 甲基化 sfrp1 |
Hypermethylation of sfrp1 gene on laterally spreading tumors |
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| ZHAI Guo-dong,WANG Xin-ying,GONG Wei,JIANG Bo.Institute for Digestive Diseases,Nanfang Hospital,Southern Medical University,Guangzhou ,China. Hypermethylation of sfrp1 gene on laterally spreading tumors[J]. Modern Digestion & Intervention, 2011, 0(2): 105-108 |
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| Authors: | ZHAI Guo-dong WANG Xin-ying GONG Wei JIANG Bo.Institute for Digestive Diseases Nanfang Hospital Southern Medical University Guangzhou China |
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| Affiliation: | ZHAI Guo-dong,WANG Xin-ying,GONG Wei,JIANG Bo.Institute for Digestive Diseases,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To investigate the expression and methylation of Wntantagonist genes on laterally spreading tumors (LSTs) and protruding adenomas (PAs).Methods We investigated the expression of key molecules (sfrp1,2,5 genes) in Wnt signaling pathway by semi-quantitative RT-PCR and immunostaining techniques.The methylation of Wnt antagonist genes in tissues (consisted of 52 LSTs and 51 PAs) was analysed by methylation specific PCR (MSP).Results The expression of sfrp1 mRNA in LST group was significantly lower tha... |
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| Keywords: | Laterally spreading tumors(LSTs) Methylation sfrp1 |
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