尿8-异前列腺素F_(2α)测定在新生儿缺氧缺血性脑病病情评估中的价值(英文)

目的：测定新生儿尿8-异前列腺素F2α(8-iso-PGF2α)水平，分析其在缺氧缺血性脑病（HIE）患儿病情评估中的价值。方法：分别于生后1 d、3 d和7d收集正常（n = 126）和HIE（n = 151）新生儿尿液，应用ELISA法检测其8-iso-PGF-2α含量。结果：①正常新生儿生后1、3和7d的尿8-iso-PGF2α水平分别为29.9±7.9、27.7±9.8和27.5±10.5 ng/mmol·Cr，差异无统计学意义；②病程第1 d，轻、中和重度HIE患儿尿8-iso-PGF2α水平分别为65.3±13.2、154.6±31.6和241.7±41.0 ng/mmol·Cr，差异具有显著性意义，且均高于正常新生儿水平(P＜0.001)；③病程第3 d，中度和重度HIE患儿8-iso-PGF2α水平（34.2±10.3ng/mmol·Cr 、50.8±12.8 ng/mmol·Cr）仍高于正常新生儿(P＜0.001），而轻度HIE患儿尿8-iso-PGF2α水平下降至正常新生儿水平；④病程第7 d，轻、中和重度HIE及正常新生儿之间的8-iso-PGF-2α水平无明显差异；⑤以8-iso-PGF2α水平45.5、89.9 和217.5 ng/mmol·Cr作为区分正常与轻度HIE、轻度与中度HIE、中度与重度的界限，其敏感性和特异性分别为95.2%和99.2%，100%和95.2%，65.8%和 100%。结论：生后7d内正常新生儿尿8-iso-PGF2α水平相对稳定；HIE患儿尿中8-iso-PGF2α峰值于病程第1 d出现，其升高程度与病情严重程度相关，是一项评估患儿病情可靠而简便的生化指标。［中国当代儿科杂志，2005, 7(2):103-106］

Value of urine 8-iso-PGF2α in the assessment of the severity of neonatal hypoxic-ischemic encephalopathy

Objective It was aimed to determine the concentration of urine 8-iso-PGF2α in normal neonates and neonates with hypoxic-ischemic encephalopathy (HIE) and to study the value of urine 8-iso-PGF2α in the assessment of the severity of neonatal HIE. Methods Urine samples from normal (n = 126) and HIE ( n = 151 ) neonates were collected on the 1 st, 3rd and 7th days after birth. ELISA was used to determine the urine 8-iso-PGF2α contents. Results 1 ) Urine 8-and 7th days after birth, respectively. There was no significant difference among the three days for the urine 8-iso-PGF2α contents. 2) On the 1 st day of HIE onset, neonates with mild, moderate and severe HIE had higher levels of urine 8-isoneonates (P < 0. 001 ). 3 ) On the 3rd day, the urine 8-iso-PGF2α content in neonates with moderate and severe HIE while that of the neonates with mild HIE regressed to normal. 4) On the 7th day, there was no significant difference in the of urine 8-iso-PGF2α were defined to distinguish from normal to mild HIE, from mild to moderate HIE and from moderate to severe HIE. As defined, the corresponding sensitivity and specificity were 95.2% and 99.2%, 100% and 95.2%,65.8% and 100%. Conclusions The urine 8-iso-PGF2α levels in neonates were stable within 7 days after birth. The urine 8-iso-PGF2α contents in HIE neonates reached the peak within 24 hours after onset. There was a correlation between the urine 8-iso-PGF2α contents and the severity of HIE, suggesting that urine 8-iso-PGF2α may be a reliable and convenient index for the assessment of the severity of HIE.