大鼠外周血淋巴细胞2 Gy γ射线照射后12 h差异基因表达谱的研究

目的 通过研究2 Gy γ射线离体和全身照后12 h大鼠外周血淋巴细胞差异基因表达谱的变化情况,在分子水平上为辐射损伤机制的研究提供依据。方法 利用基因芯片技术对大鼠离体和全身照射的外周血淋巴细胞进行辐射差异基因筛选和Pathway分析,并利用荧光定量PCR技术对基因芯片结果进行验证。结果 离体照射组筛选出差异3倍以上的基因2 001条,全身照射组筛选出差异3倍以上的基因2 590条。两组共同差异3倍以上的基因有312条。离体照射组涉及22个KEGG通路,全身照射组涉及35个KEGG通路,两组有10个共同的KEGG通路。选择其中3条基因进行实时荧光PCR定量分析,得到的相对定量结果与芯片检测结果趋势一致。结论 从照后12 h的研究结果中发现,差异表达基因涉及细胞凋亡、细胞周期及信号转导等多个方面,这将为深入研究这些辐射相关基因在淋巴细胞辐射损伤中的作用提供依据。

Study of Differential Gene Expression Profiling in Rat Peripheral Blood Lymphocyte 12 Hours after in-vitro and Whole body Exposure to 2Gy Gamma Ray

Objective To evaluate the changes of differential gene expression in peripheral blood lymphocyte of SD rats 12 hours after in-vitro and whole-body exposure to 2Gy ⁶⁰Co γ-ray, and provide the evidence for irradiation damage in gene level. Methods The method of microarray was applied to detect the differentially expressed genes and analyze the pathways. Gene expression was measured using real-time quantitative PCR to validate the results of the microarray. Results The results showed that there were 2 001 genes differentially expressed over 3 fold in in-vitro group, 2 590 genes in whole-body group, and 312 genes in both in-vitro and whole-body groups differentially expressed. There were 22 KEGG pathways in in-vitro group, 35 KEGG pathways in whole-body group, and 10 KEGG pathways in both. The relative quantity's results of three genes, selected to validate the microarray, were consistent with microarray results. Conclusion These results indicate that the differentially expressed genes are related to apoptosis, cell cycle and signal transduction etc, which will provide a basis for the further study of mechanism of irradiation damage.