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氨基酸、有机酸、碱基核苷类化合物的双向色谱多相同步分析
引用本文:吴有光,匡渤海,等.氨基酸、有机酸、碱基核苷类化合物的双向色谱多相同步分析[J].江西医学院学报,1994,34(4):1-6,F002.
作者姓名:吴有光  匡渤海
作者单位:医学遗传研究室,南昌330006
摘    要:30余种氨基酸、40余种非挥发性有机酸和20余种碱基核苷类化合物在一块薄层板上完成多相同步色谱分析;用紫外灯-吖啶试剂-镉茚三酮试剂实行组合显色定位分析。改善了尿液中非挥发性有机酸的溶剂萃取法,尿中三类化合物提取物的同步多相色谱分离效果良好。

关 键 词:氨基酸  有机酸  核苷  薄层色谱法  同步多相分析  尿液检验

Two Dimensional Thin Layer Chromatographyfor Synchronous Analyses of Amino Acids Organic AcidsNucleosides and Purine and Pyrimidine Bases on a sheet
Wu Youguang, Kuang Bohai, Waug Anan, Long Fei.Two Dimensional Thin Layer Chromatographyfor Synchronous Analyses of Amino Acids Organic AcidsNucleosides and Purine and Pyrimidine Bases on a sheet[J].Acta Academiae Medicinae Jiangxi,1994,34(4):1-6,F002.
Authors:Wu Youguang  Kuang Bohai  Waug Anan  Long Fei
Abstract:Ovre thrty amino acids. 40 non-volatile organic acids and 20 nucleosides and related compouds have been synchronously separated by two dimensional thin tayer chromatographyon a plate. The localisation of the separated compound spots was performed with multipleprocedures:viewing the absorbing spots in U. V light(254mm)-colouring in acridinereagents for most organic acids-colouring by ninhydrin-acetate cadmium for amino acids.The method of solvent extraction for the non-volatole organic acids in urine was improved.And the effect of separating of the metabolites of three componnds in urine on thin layerplates was satisfactorv. This method enables us to screen the inborn errors of amino acid,or-ganic acid and purine and pyrimidine metabolism in the primary hospitals simuItanously.
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