徐长卿丹皮酚对兔膝骨性关节炎软骨细胞凋亡的影响

探讨徐长卿丹皮酚对兔膝骨性关节炎软骨细胞凋亡及相关调控蛋白Bcl-2,Bax表达的影响。采用兔膝关节内侧半月板前1/3切除及前交叉韧带切断制备骨关节炎(OA)模型。于OA模型建立后的第1周开始对右膝关节内分别注射徐长卿丹皮酚0.2 mg、曲安奈德0.2 mg、0.9%生理盐水0.3 mg,每周2次,连续给药5周。用药结束1周后取关节软骨标本制作切片,透射电镜观察软骨细胞凋亡的结构变化;TUNEL法检测软骨细胞凋亡,计算软骨细胞凋亡指数;免疫荧光法检测软骨细胞Bcl-2和Bax的表达。结果显示徐长卿组和曲安奈德组的凋亡率与对照组相比均显著降低(P<0.05,P<0.01),徐长卿丹皮酚和曲安奈德均能上调Bcl-2蛋白的表达,下调Bax蛋白的表达,从而升高Bcl-2/Bax的比值,进而抑制细胞凋亡,其中徐长卿丹皮酚与模型组比较,升高Bcl-2/Bax的比值更为显著(P<0.01)。

Effects of Cynanchum paniculatum paeonol on apoptosis of articular chondrocyte in rabbits with knee osteoarthritis

To explore the effects of Cynanchum paniculatum paeonol on apoptosis of articular chondrocyte and the expression of Bcl-2 and Bax in rabbits with knee osteoarthritis(OA), the rabbit model was established by anterior cruciate ligament transaction and excision of partial medial meniscus. From the first week after the OA model established, Cynanchum paniculatum paeonol, triamcinolone acetonide and 0. 9% saline were injected in the right knee with 0. 2, 0. 2 and 0. 3 mg, respectively, twice a week for 5 weeks. Articular cartilage samples were taken and sliced one week after the end of treatment. The morphological changes of cells were observed under electronic microscope; the TUNEL method was employed to observe the apoptosis of articular chondrocyte; and the immunofluorescence assay was used to detect theexpression of Bcl-2 and Bax inchondrocytes. The results showed that the apoptosis rates in Cynanchum paniculatum paeonol group and triamcinolone acetonide group were significantly lower than that of the control group(P< 0. 05, P< 0. 01). Cynanchum paniculatum paeonol and triamcinolone acetonide could increase the expression of Bcl-2 and decrease the expression of Bax, thus raising the ratio of Bcl-2/Bax and inhibiting apoptosis, and the Bcl-2/Bax ratio in Cynanchum paniculatum paeonol group was much higher than that of model group(P< 0. 01).