Some technical improvements for the voltage clamp with the double sucrose gap |
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Authors: | C Léoty J Alix |
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Institution: | (1) Laboratoire de Physiologie Animale et de Physiologie Cellulaire des Structures Contractiles (C.N.R.S.: E.R.A. no. 111), Université de Poitiers, 40, Avenue Recteur Pineau, F-86022 Poitiers Cédex, France |
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Abstract: | Summary A new double sucrose gap chamber using electrodes of high stability and low resistance is described. The size of each sucrose gap and the central node can be modified independently so that this chamber is suitable for research on multicellular or unicellular excitable preparation under voltage clamp conditions. The shape of the central channel is made so as to reduce the series resistance between the preparation and the recording electrode to a minimum. A window made in the floor of the chamber allows to observe the preparation under a microscope.This work was performed under the I.N.S.E.R.M. grant no. 17.75.40. |
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Keywords: | Double sucrose gap technique Electrodes Voltage clamp |
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