| DAPK诱导PGCl3细胞凋亡时Bcl-2磷酸化的研究 |
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| 引用本文： | 伍俊,梁标,张海涛.DAPK诱导PGCl3细胞凋亡时Bcl-2磷酸化的研究[J].中国癌症杂志,2005,15(5):470-472. |
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| 作者姓名： | 伍俊 梁标 张海涛 |
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| 作者单位： | 广东医学院附属医院呼吸疾病研究所，广东医学院附属医院呼吸疾病研究所，广东医学院生化教研室 广东湛江524001 |
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| 摘 要： | 目的:探讨DAPK诱导PGCl3细胞凋亡的可能机制。方法:真核表达载体pcDNA3.1-DAPK导入PGCl3细胞中。RT-PCR检测bcl-2和baxm-RNA丰度变化,Bcl-2含量变化和Bcl-2磷酸化水平变化。结果:DAPK诱导PGCl3细胞凋亡时,bcl-2表达是下调的,bax基因表达量无明显变化,pcDNA3.1-DAPK转染组的Bcl-2磷酸化水平相对pcDNA3.1组要高,同时Bcl-2蛋白含量是减少的。结论:过表达的DAPK诱导PGCl3细胞凋亡与bcl-2转录下调和Bcl-2磷酸化有关
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| 关 键 词： | 死亡相关蛋白激酶 bcl-2 凋亡 |
| 文章编号： | 1007-3639(2005)05-0470-03 |
| 收稿时间： | 2005-05-16 |
| 修稿时间： | 2005-07-14 |
Study on changes of bcl-2 expression in apoptosis of PGCl3 cells induced by DAPK |
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| WU Jun,LI ANG Biao,ZHANG Hai-tao.Study on changes of bcl-2 expression in apoptosis of PGCl3 cells induced by DAPK[J].China Oncology,2005,15(5):470-472. |
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| Authors: | WU Jun LI ANG Biao ZHANG Hai-tao |
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| Institution: | Institute of Respiration,Affiliated Hospital of Guangdong Medical College,Zhanjiang Guangdong,524001,China |
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| Abstract: | Purpose:To investigate effect on bcl-2 and bax expression in apoptic PGCl_(3) cells and to study the mechanism of apoptosis of PGCl_(3) cells induced by death associated protein kinase(DAPK).Methods:Eukaryotic express vector pcDNA3.1-DAPK was tranfected into High-metastasis Non-small Lung Cancer Cell PGCl_(3).Changes of bcl2 and bax expression were detected with RT-PCR.Phospharation of Bcl-2 was examined with Westernblotting.Results:It was demonstrated that bcl-2 expression was down regulated,but bax expression did not change in apoptosis of PGCl_(3) cells induced by DAPK.Level of Phospharation of Bcl-2 increased.Conclusions:Apoptosis of PGCl_(3) cells induced by over-expression of DAPK may be associated with down regulating of bcl-2 expressio and increase of phospharation of Bcl-2. |
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| Keywords: | death associated protein kinase bcl-2 apoptosis |
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