| 骨肉瘤细胞凋亡相关基因的表达及其临床意义 |
| |
| 引用本文： | Wu X,Chen ZR,Zhang GJ. 骨肉瘤细胞凋亡相关基因的表达及其临床意义[J]. 中华肿瘤杂志, 2004, 26(11): 678-681 |
| |
| 作者姓名： | Wu X Chen ZR Zhang GJ |
| |
| 作者单位： | 1. 200072,上海市第十人民医院骨科
2. 200032,上海,复旦大学附属中山医院骨科 |
| |
| 摘 要： | 目的 探讨反映骨肉瘤预后的蛋白标记物。方法 对骨肉瘤石蜡组织作免疫组化和Tunel染色,研究p53 c-myc和bcl-2基因的表达与肿瘤细胞凋亡指数(Al)的关系,及其与病理类型和患者颅后的关系。结果 p53、c-myc和bcl-2的表达水平与细胞凋亡指数呈负相关,与病理类型无相关关性。p53、c-myc、bcl-2和细胞凋亡指数与患者远期生存密切相关。结论 p53、c-myc和bcl-2的表达水平以及细胞凋亡指数可作为判断骨肉瘤恶性程度和预后的指标,可指导临床进行治疗。
|
| 关 键 词： | 骨肉瘤 细胞凋亡 免疫组化 基闪表达 |
Apoptosis-related gene expression and its clinical significance of human osteosarcoma |
| |
| Wu Xing,Chen Zheng-rong,Zhang Guang-jian. Apoptosis-related gene expression and its clinical significance of human osteosarcoma[J]. Chinese Journal of Oncology, 2004, 26(11): 678-681 |
| |
| Authors: | Wu Xing Chen Zheng-rong Zhang Guang-jian |
| |
| Affiliation: | Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai 200032, China. wuxinggood@163.com |
| |
| Abstract: | Objective To explore the prognostic markers in osteosarcoma. Methods Expressions of p53, c-myc, bcl-2 and apoptosis index (AI) in 28 osteosarcoma specimens were detected by ABC immunohistochemistry and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end labelling (TUNEL). The relationship between gene expression and apoptosis, and their correlations with pathologic classification and prognostic factors were analyzed. Results There was negative correlation between the expressions of p53, c-myc, bcl-2 protein and AI, which was closely related to the long term survival of patients but was not related to pathologic types of the tumor. Conclusion The expressions of p53, c-myc, bcl-2 protein and AI can be used as an index for predicting the progression and prognosis of osteosarcoma. |
| |
| Keywords: | Osteosarcoma Gene p53 Gene c-myc Gene bcl-2 Apoptosis Gene expression |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
|
