副溶血性弧菌160株血清群型分布和耐药性分析

[目的]了解上海市浦东新区副溶血性弧菌群型分布及耐药情况。[方法]采用血清玻片凝集试验对160株副溶血性弧菌进行血清分型;采用改良K-B法进行耐药性测定。[结果]59株食物中毒分离株完全分型56株,分型率为94.92%,共分出7种血清型,O3∶K6(77.97%)居首位。101株水产品分离株完全分型32株,分型率为31.68%,共分出22种血清型,无优势血清型。药敏试验显示,副溶血性弧菌对氨苄西林高度耐药,对阿莫西林/克拉维酸、头孢吡肟、头孢噻肟、头孢他啶、氯霉素100.00%敏感。[结论]O3∶K6型是引起本区副溶血性弧菌食物中毒的主要血清型;水产品分离株血清型呈多样性,与食物中毒分离株不一致。本地区副溶血性弧菌没有产生广泛的耐药性变异。

Analysis of 160 serotypes and drug resistance of Vibrio parahaemolyticus

[Objective] To ascertain the serotype and the drug resistance of Vibrio parahaemolyticus in Pudong New Area of Shanghai. [Methods] The slide serum agglutination test was used to detect the 160 serotypes of Vibrio parahaemolyticus and the K-B method was used to test the drug resistance. [Results] In 59 strains isolated from food poisoning,7 serotypes were typed in 56 strains.The total typing rate was 94.92% and the main serotype was O3∶K6 accounting for 77.97%.In 101 strains isolated from aquatic products,22 serotypes with no popular type were typed in 32 strains and the total typing rate was 31.68%.The test of drug resistance showed high resistance to Ampicillin and 100.00% sensitive to Amoxicillin/clavulanic acid,Cefotaxime,Ceftazidime,Cefepime and Chloramphenicol. [Conclusion] The main serotype of Vibrio parahaemolyticus from food poisoning strains in Pudong was O3∶K6.The serotypes isolated from aquatic products were diverse and different from the serotypes of food poisoning strains.Vibrio parahaemolyticus in Pudong did not mutate widely in drug resistance test.