采用高效液相色谱法测定薯片及饼干中丙烯酰胺的研究

目的]建立测定薯片及饼干中丙烯酰胺含量的高效液相色谱方法。方法]采用纯水作提取剂,样品经正己烷去脂、高速冷冻离心、过SPE小柱净化后,进行高效液相色谱分析。结果]该方法标准曲线的相关系数为0.9995,平均加标回收率为98.2%,相对标准偏差(RSD)为7.8%,方法的检出限和定量检出限分别为10μg/kg和31μg/kg。结论]该方法操作简便快捷、经济、灵敏度高,适用于一般实验室开展日常食品检测和监测。

Determination of acrylamide in potato chips and biscuits by high performance liquid chromatography

Objective] To develop an approach to determining acrylamide in potato chips and biscuits by high performance liquid chromatography(HPLC).Methods] The acrylamide in the samples was extracted by ultrapure water and cleaned up by centrifugation,freezing and solid phase extraction,then analyzed by HPLC.Results] The study showed the correlation coefficient was 0.9995.The average spike recovery rate and the relative standard deviation(RSD) were 98.2% and 7.8% respectively.The limit of detection(LOD) and the limit of quantification(LOQ) were 10 μg/kg and 31 μg/kg.Conclusion] The approach is proved to be simple,convenient,economic,sensitive,and applicable in laboratories to monitoring and inspection of daily foods.