骨髓基质干细胞复合纳米羟基磷灰石/聚乳酸构建组织工程骨

目的：了解骨髓基质干细胞（MSCs）复合纳米羟基磷灰石／聚乳酸（n-HA／PLA）构建组织工程骨的异位成骨作用。方法：选择6只新西兰白兔，实验组于动物脊柱左侧肌肉内植入MSCs复合n-HA／PLA构建的组织工程骨，对照组于右侧植入n-HA／PLA生物材料。术后4、8周取材，行溴脱氧尿嘧啶（Brdu）标记细胞检测和组织学观察。结果：术后4周，实验组材料边缘均可检测到Brdu标记的MSCs。术后4、8周，实验组材料内部有新骨形成，随着时间推移，新骨量增多，编织骨向板层骨过渡。对照组材料未见新骨形成。结论：MSCs复合n-HA／PLA构建的组织工程骨具有很好的异位成骨作用。

The fabrication of tissue engineering bone with bone marrow stromal clls and nanohydroapatite / polylactic acid

Objective To study the effect of ectopic osteogenesis of fabrication of tissue engineering bone with bone marrow stromal cells (MSCs) and nano-hydroapatite / polylactic acid(n-HA/PLA). Methods The materials were implanted in bilateral muscle of spine in 6 New Zealand white rabbits which were divided into experiment and control group. Tissue engineering bone was implanted in left muscle of spine in experiment group, while simple biomaterial in right muscle of spine in control group. When the samples were harvested at 4, 8 weeks postoperatively, a series of examination were carried out, including the bromodeoxyuridine (Brdu) labelled cells detection and histomorphological observation. Results The Brdu labelled cells were detected around the edge of materials in experiment group. The woven bone reconstructed into lamellar bone. There was no new bone formation in control group. Conclusion The fabrication of tissue engineering bone with MSCs and n-HA/PLA had good effect of ectopic osteogenesis.