C57BL/6J小鼠脂肪细胞抵抗胰岛素抑制脂解的分析

为了从细胞水平探讨Ｃ５７ＢＬ／６Ｊ和Ａ／Ｊ小鼠对胰岛素（Ｉｎｓ）的敏感性，该实验分离了这两种小鼠的脂肪细胞，观察Ｉｎｓ 抑制脂肪细胞在异丙肾上腺素介导下的脂解作用，以甘油的释放量作为脂解的指标。结果表明：Ｃ５７ＢＬ／６Ｊ的脂肪细胞在Ｉｎｓ ４００和８００ ｐｍ ｏｌ时甘油的释放量多于Ａ／Ｊ（Ｐ＜ ０．０１），即Ｉｎｓ抗脂解能力显著低于Ａ／Ｊ小鼠，提示Ｃ５７ＢＬ／６Ｊ小鼠脂肪细胞抵抗Ｉｎｓ抑制脂解。这与Ｃ５７ＢＬ／６Ｊ为什么易于发生２ 型糖尿病可能存在病理生理方面的联系。

Insulin-ResistantLipolysis in Adipocytes Isolated from C57BL/6J Mice

It has been demonstrated that C57BL/6J mice is prone to develop non insulin dependent diabetes mellitus (NIDDM),but A/J mice isn't.NIDDM is related to insulin resistance.However,it is unknown whether C57BL/6J mice exist insulin resistance before developing NIDDM.In order to investigate insulin sensitivity from cellular level,we assessed the effect of insulin inhibiting the isoproterenol induced lipolysis in adipocytes isolated from C57BL/6J and A/J mice.Glycerol released from adipocyte medium was used as the index of lipolysis,Glycerol concentration was significantly higher in adipocytes from C57BL/6J than that from A/J at 400 and 800 pmol insulin in medium( P <0 01).That meant the ability of insulin inhibiting lipolysis was lower in adipocyte of C57BL/6J than in that of A/J.Resistance to the antilipolytic action of insulin in adipocyte from C57BL/6J may be associated with the susceptibility to develop NIDDM of C57BL/6J mice in pathophysiology.