多指标均匀设计法优选痤疮消凝胶提取工艺

目的:优选痤疮消凝胶的提取工艺,为该制剂的临床应用提供参考。方法:以大黄游离总蒽醌、栀子苷、咖啡酸含量的综合评分为指标,采用均匀设计法考察乙醇体积分数、乙醇用量、提取时间和提取次数对提取工艺的影响。采用HPLC测定大黄游离总蒽醌、栀子苷、咖啡酸含量,流动相分别为甲醇-0.1%磷酸溶液(85:15),甲醇-水(23:77),乙腈-0.1%磷酸溶液(10:90),检测波长依次为254,238,323 nm。结果:最佳提取工艺参数为加12倍量80%乙醇提取3次,每次1 h。大黄游离总蒽醌、栀子苷、咖啡酸质量浓度分别为1 260.33,1 597.56,32.19 mg·L^-1。结论:优选的痤疮消凝胶提取工艺合理可行、重复性好,为该制剂生产工艺参数的确定提供实验依据。

Optimization of Extraction Process of Cuochuangxiao Gel by Uniform Design

Objective: To optimize extraction technique of Cuochuangxiao gel and provide a reference for clinical application of this preparation. Method: With composite score of contents of free anthraquinones in Rhei Radix et Rhizoma, gardenin and caffeic acid as index, uniform design was adopted to investigate effects of ethanol concentration, ethanol volume, extraction time and times on extraction process of Cuochuangxiao gel.HPLC was employed to determine contents of free anthraquinones in Rhei Radix et Rhizoma, gardenin and caffeic acid with mobile phases of methanol-0.1% phosphoric acid (85:15), methanol-water (23:77) and acetonitrile-0.1% phosphoric acid (10:90), detection wavelengthes of 254, 238, 323 nm, respectively. Result: Optimum extraction conditions were as follows:added 12 times the amount of 80% ethanol, extracted 3 times for 1 h each time.Under these conditions, contents of free anthraquinones in Rhei Radix et Rhizoma, gardenin and caffeic acid were 1 260.33, 1 597.56, 32.19 mg·L^-1, respectively. Conclusion: This extraction process is reasonable, stable and feasible, which provides a theoretic basis for industrial production of Cuochuangxiao gel.