Melatonin ameliorates bisphenol A-induced biochemical toxicity in testicular mitochondria of mouse |
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Authors: | Sameya Anjum Shakilur Rahman Manpreet Kaur Firoz Ahmad Hina Rashid Rizwan Ahmad Ansari Sheikh Raisuddin |
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Institution: | aDepartment of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi 110062, India;bDepartment of Veterinary and Biomedical Sciences and the Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, University Park, PA 16802, USA |
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Abstract: | Bisphenol A (BPA) is a monomer of polycarbonate plastic used to manufacture plastic baby bottles and lining of food cans. It has endocrine-disrupting potential and exerts both toxic and estrogenic effects on mammalian cells. We studied BPA-induced perturbation of mitochondrial marker enzymes in testes of Swiss albino mice and its amelioration by melatonin. Mice exposed to standardized dose of BPA (10 mg/kg body weight) orally for 14 days showed decrease in activities of marker mitochondrial enzymes such as succinate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, monoamine oxidase and NADH dehydrogenase. Besides, it also affected activities of antioxidant enzymes such as superoxide dismutase, glutathione reductase and glutathione peroxidase. BPA also caused lipid peroxidation (LPO) and decrease in reduced glutathione (GSH) content of mitochondria. Concomitant melatonin administration (10 mg/kg body weight; intraperitoneally for 14 days) lowered mitochondrial lipid peroxidation. It also restored the activity of mitochondrial marker enzymes and ameliorated decreased enzymatic and non-enzymatic antioxidants of mitochondria. These results demonstrate that melatonin has a potential role in ameliorating BPA-induced mitochondrial toxicity and the protection is due to its antioxidant property or by the direct free radical scavenging activity. |
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Keywords: | Abbreviations: ANOVA analysis of variance BPA bisphenol A EDTA ethylenediamine tetra acetic acid GPx glutathione peroxidase GR glutathione reductase GSH reduced glutathione IDH isocitrate dehydrogenase LPO lipid peroxidation MAO monoamine oxidase MDH malate dehydrogenase NADH nicotinamide adenine dinucleotide reduced NDH NADH dehydrogenase RNS reactive nitrogen species ROS reactive oxygen species SDH succinate dehydrogenase TBARS thiobarbituric acid reactive substances |
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