碱性成纤维细胞生长因子对不同年龄白内障患者晶状体上皮细胞增殖的影响

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The age-related effect of basic fibroblast growth factor on the proliferation of lens epithelial cells

OBJECTIVE: To detect the age-related effect of basic fibroblast growth factor (bFGF) on the proliferation of human lens epithelial cells (HLECs) in patients with cataract and discuss the mechanism. METHODS: Human lens anterior capsules with attached HLECs were cultured in vitro, treated with 10 microg/L bFGF for 48hr, and then the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry was estimated. Immunohistochemical analysis of bFGF receptor was also carried out. RESULTS: The bFGF could enhance the HLEC proliferation in patients with cataract at various ages. The enhancement of the proliferation and the expression of bFGF receptor protein were negatively correlated to the age of the patients with cataract (P < 0.01). CONCLUSIONS: The basic fibroblast growth factor might play an important role in the formation of posterior capsular opacity (PCO) after cataract surgery. With the decrease in the patient's age, the enhancement of HLEC proliferation is increased, indicating that the fact is related to the high incidence of PCO in children.