慢性HCV感染与机体细胞免疫功能关系的探讨

目的:探讨慢性丙型肝炎病毒感染患者HCV RNA增殖状态与T细胞亚群功能及血浆中IL-2和SIL-2R活性的关系。方法:以间接免疫荧光法、ELISA法和RT-PCR分别检测75例慢性HCV感染患者(其中HCV RNA阳性47例,HCV RNA阴性28例)外周血T细胞亚群、IL-2、sIL-2R的水平和HCV RNA。结果:慢性HCV感染患者周围血CD_3~ 、CD_4~ 淋巴细胞亚群,CD_4~ /CD_3~ 比值及IL-2水平均显著低于正常对照组(P<0.01),而sIL-2R明显升高(P<0.01);血清HCV RNA阳性患者T细胞亚群,CD_4~ /CD_3~ 比值及IL-2水平显著低于HCV RNA阴性患者(P<0.01)。结论:慢性HCV感染患者存在机体细胞免疫功能低下,HCV RNA阳性患者较阴性患者更甚,提示细胞免疫功能受抑可能是HCV持续增殖的原因。

Study on Relationship between Immune Function and Chronic HCV Infection State

Background/Aims: To investigate the relationship between T lymphocyte subsets, serum levels of IL-2, sIL-2R and HCV RNA proliferation state. Methods: Peripheral blood of 75 patients were determined for T lymphocyte subsets by indirect immnofluo rescence, levels of IL-2 and sIL-2R by indirect immunofluorescence and ELISA and HCV RNA by RT-PCR respectively. 47 cases were HCV RNA postive, 28 were negative. Results: CD3 , CD4 T lymphocyte subsets levels, CD4 /CD8 ratio and serum IL-2 levels were significantly lower in patients than in healthy controls (P<0.01), sIL-2R levels increased significantly (P<3.01). T lymphocyte subsets, CD4 /CD8 ratio and IL-2 levels were significantly lower in HCV RNA positive patients than in HCV RNA negative patients (P<0.01). Conclusions: Patients with chronic HCV infection may have cellular immune dysfunction, HCV RNA positive patients were more obvious than HCV RNA negative. These results suggested that cellular immune suppression may play an important role in HCV RNA persistant proliferation.