Interaction between gene p53 and oncogene mdm2 in human glandular lung cancer cell line GLC-82

Objective To study the interaction between oncogene mdm2 and wp53 in human glandular lung cancer cell line GLC 82.MethodsBy lipofectamine mediated DNA transfec~tion, wp53 and mdm2 were transfected separately or co transfected into GLC 82 cells via retrovival vector pDOR neo, a carrier of wp53 and mdm2.Results The growth of GLC 82 cells was blocked and their DNA synthesis inhibited by wp53, its colony forming rate in soft agar culture and the tumorigenicity in nude mice declined and mdm2 antagonized the function of wp53.Conclusion After the recombinant vector pDOR mdm2 was transfected into GLC 82 cells containing wp53, mdm2 partially deprives wp53 of its function of inhibiting the growth of GLC 82 cells.