| HSV-TK基因在膀胱癌小鼠体内的转移和丙氧鸟苷治疗效果 |
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| 引用本文： | 刘为池,叶钢,张荣贵. HSV-TK基因在膀胱癌小鼠体内的转移和丙氧鸟苷治疗效果[J]. 南方医科大学学报, 2002, 22(1): 41-42,44 |
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| 作者姓名： | 刘为池 叶钢 张荣贵 |
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| 作者单位： | 第三军医大学新桥医院泌尿外科,重庆,400037;第三军医大学新桥医院泌尿外科,重庆,400037;第三军医大学新桥医院泌尿外科,重庆,400037 |
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| 基金项目： | 重庆市科委基金(1997-53) |
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| 摘 要： | 目的探讨HSV-TK基因在膀胱癌动物体内的转移途径,观察HSV-TK/GCV(丙氧鸟苷)系统体内治疗的效果。方法在T739同基因鼠膀胱癌皮下肿瘤模型中,以裸质粒DNA瘤内直接注射、脂质体包裹和逆转录病毒介导等3种不同方法转移HyTK基因,观察丙氧鸟苷对膀胱癌的治疗效果。结果脂质体复合物组及逆转录病毒组背部肿瘤生长减慢,抑瘤率分别为54%、68%;动物平均存活时间分别比对照组延长28.81%、44.16%。结论脂质体、逆转录病毒介导的基因转移是膀胱癌基因治疗的有效手段。
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| 关 键 词： | 膀胱癌 基因治疗 脂质体 逆转录病毒 单纯疱疹病毒-胸苷激酶 |
| 文章编号： | 1000-2588(2002)01-0041-02 |
| 修稿时间： | 2001-09-28 |
In vivo HSV-TK gene transfer and the therapeutic effects of ganciclovir on bladder cancer in mice with HSV-TK gene transfer |
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| LIUWei-chi,YEGang,ZHANGRong-gui Departmentof Urology,XinqiaoHospital,ThirdMilitaryMedicalUniversity,Chongqing,China. In vivo HSV-TK gene transfer and the therapeutic effects of ganciclovir on bladder cancer in mice with HSV-TK gene transfer[J]. Journal of Southern Medical University, 2002, 22(1): 41-42,44 |
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| Authors: | LIUWei-chi YEGang ZHANGRong-gui Departmentof Urology XinqiaoHospital ThirdMilitaryMedicalUniversity Chongqing China |
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| Affiliation: | LIUWei-chi,YEGang,ZHANGRong-gui Departmentof Urology,XinqiaoHospital,ThirdMilitaryMedicalUniversity,Chongqing400037,China |
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| Abstract: | Objective To explorean effectivemethodforin vivo HSV-TKgenetransferandobservethetherapeuticeffectof ganciclovir(GCV)on bladdercancerin miceafterreceivingsuchgenetransfertherapy.Methods Micemodelsbearing subcutaneoustumorsof bladdercancerwereestablishedin24syngeneicmice,andHSV-TKgenetransferwasperformedby meansof nakedDNA plasmids,DNA-liposomecomplexor retroviralmediatorsrespectively.GCV treatmentwas administeredin allthemiceanditseffecton thegrowthof thetumorwas subsequentlyobserved.Results Genetransfer therapiesby DNA-liposomecomplexandretroviralmediatorsbothsignificantlyinhibitedthegrowthof thetumorby54%and68%respectively,andthesurvivalof themicewasprolongedby28.81%and44.16%(P<0.05).Theeffectof genetransferby nakedDNA plasmidswas not obvious(P>0.05).Conclusion Genetransfertherapiesby DNA-liposomecomplexand retroviralvectorssupplementedby GCVtreatmentareeffectivetherapeuticmodalitiesagainstbladdercancer. |
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| Keywords: | bladdercancer genetherapy liposome retrovirus herpessimplexvirusthymidinekinase |
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