内蒙古包头地区汉族人群TNF-α-308位点基因多态性与IgA肾病的相关性研究

目的：了解内蒙古包头地区汉族人群IgA肾病患者肿瘤坏死因子α（TNF-α）-308位点基因型的分布特点。方法：应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测97例IgA肾病患者和73例正常人的TNF-α基因型。结果：IgA肾病患者组中TNF-α基因G/A多态GG、GA、AA基因型频率分别为85.6%、11.3%和3.1%,其中G和A等位基因频率分别为91.2%和8.8%。正常对照组中,TNF-α基因G/A多态GG、GA、AA基因型频率分别为91.8%、5.5%和2.7%,其中G和A等位基因频率分别为94.5%和5.5%。IgA肾病患者组与正常对照组比较,基因型频率差异有显著性（χ^2=27.2,P〈0.05）,等位基因频率之间差异无显著性（χ^2=0.659,P〉0.05）。结论：TNF-α-308位点基因多态性与内蒙古包头地区汉族人群IgA肾病的发病有相关性,等位基因频率之间差异无显著性（χ^2=0.659,P〉0.05）。

Study on the Relationship Between Tumor Necrosis Factor-α (TNF-α) Gene-308 Site Polymorphism and IgA Nephropathy in Ethnic Han Population in Baotou Region of Inner Mongolia

Objective To study the relationship between tumor necrosis factor -α（TNF -α） gene -308 site polymorphism and IgA nephropathy in ethnic Han population in Bac tea region of Inner Mongolia. Methods The polymorphism of TNF - α gene was studied with PCR - RFLP in 97 patients with IgA nephropathy and 73 controls. Results The frequencies of TNF - α gene genotypes CG, CA, AA were 85.6%, 11.3% and 3.1% respectively in patients with IgA nephropathy. The frequencies of alleles G and A were 91.2% and 8.8%. The frequencies of TNF - α gene genotypes CG, GA, AA were 91.8%, 5.5% and 2.7% in controls.The frequencies of alleles G and A were 94.5% and 5.5%. The distribution of G/A polymorphism of TNF -α gone was in agreement with Hyrdy - Weinberg equilibrium. The frequencies of TNF -α gene genotypes in patients with IgA nephropathy were significantly different （χ^2 = 27.2,P 〈 0. 05） from those in the controls. The frequencies of alleles G and A were not significantly different （χ^2 = 0.659,P 〉 0.05） from those in the controls. Conclusion TNF -α gone - 308 polymorphism is associated with IgA nephropathy, A allele may not be a risk factor in the development of IgA nephropathy in ethnic than population in Bao tou region of Inner Mongolia.