High-performance liquid chromatographic determination of diclofenac in human plasma after solid-phase extraction. |
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Authors: | C Arcelloni R Lanzi S Pedercini G Molteni I Fermo A Pontiroli R Paroni |
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Affiliation: | Department of Laboratory Medicine, Scientific Institute H S. Raffaele and School of Medicine, Milan, Italy. |
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Abstract: | A novel high-performance liquid chromatographic (HPLC) method for the quantification of diclofenac in human plasma was set up. Samples, added with ibuprofen (used as internal standard) were purified by solid-phase extraction using Abselut Nexus cartridges (Varian) not requiring pre-conditioning. Drugs of interest were eluted directly into the autosampler vials and injected. The recovery of diclofenac was 92%, the analysis lasted 7 min with a sensitivity of 5 ng/ml and intra- and inter-day RSDs of 3 and 8%, respectively. The pharmacokinetics of diclofenac after oral and rectal administration in 10 healthy volunteers are reported. |
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