Catecholamines modulate growth and differentiation of human preosteoclastic cells |
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Authors: | U Frediani L Becherini L Lasagni A Tanini M L Brandi MD PhD |
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Institution: | (1) Endocrine Unit, Department of Clinical Physiopathology, University of Florence, Viale Pieraccini 6, I-50139 Florence, Italy;(2) Endocrine Unit, IIIrd Institute of Internal Medicine, University of Florence, Florence, Italy |
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Abstract: | Using a clonal cell line of human osteoclast precursors (FLG 29.1 cells), that after treatment with 12-O-tetradecanoyl phorbol 13-acetate (TPA) show many functional characteristics of osteoclasts, we demonstrated that catecholamines act as inducers of osteoclast maturation in vitro and as stimulators of osteoclast activity via the binding to 2 adrenergic receptors. Scatchard analysis of125I-labelled iodocyano-pindolol to untreated (undifferentiated) or TPA-treated (differentiated) FLG 29.1 cells revealed the presence of a single high-affinity site with aK
d
value around 24 pM and 8 pM respectively and with superimposable binding capacity (1.18 fmol/mg protein). Catecholamines increased in a dose-dependent fashion the intracellular cyclic AMP (cAMP) accumulation in both undifferentiated and TPA-treated FLG 29.1 cells. Pretreatment of untreated and TPA-treated FLG 29.1 cells with propranolol inhibited the catecholamine effect on cAMP accumulation, while pretreatment with clonidine had no effect. Catecholamines also reduced cell proliferation, increased tartrate-resistant acid phosphatase (TRAcP) activity, interleukin 6 (IL-6) production, multinuclearity and response to salmon calcitonin (sCT) in undifferentiated FLG 29.1 cells. In differentiated FLG 29.1 cells only IL-6 release was induced by catecholamine treatment. These findings support a potential role for catecholamines in modulating osteoclast differentiation and mature osteoclast activity. |
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Keywords: | -Adrenergic receptors" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-Adrenergic receptors Epinephrine Norepinephrine Osteoclastic cells Preosteoclastic cells |
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