| 人参皂苷Rg1、Rb1对淀粉样前体蛋白分泌酶代谢途径的影响 |
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| 引用本文： | 魏翠柏,贾建平,王芬,贾龙飞.人参皂苷Rg1、Rb1对淀粉样前体蛋白分泌酶代谢途径的影响[J].中国中医药信息杂志,2008,15(9):28-30. |
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| 作者姓名： | 魏翠柏 贾建平 王芬 贾龙飞 |
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| 作者单位： | 首都医科大学宣武医院神经内科,北京,100045 |
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| 摘 要： | 目的观察人参皂苷Rg1、Rb1对淀粉样前体蛋白代谢途径中α分泌酶活性和基因表达的影响。方法用转染突变型PSl（ML46L）和野生型PSl的CHO细胞，通过荧光酶标技术检测α分泌酶活性，Westernblot免疫印迹、RT-PCR技术检测α分泌酶基因ADAM9、ADAM10的蛋白和RNA表达。结果转染突变型PSlML46L组a分泌酶活性较转染野生型PS1（WT组）低（P〈0．05），加用人参皂苷Rg1、Rb1后转染突变型PSlML46L组α分泌酶活性较未加用组不同程度增高（P〈0．05），同时ADAM9、ADAM10的蛋白及RNA表达较未加用组有不同程度增多（P〈0．05）。结论人参皂苷Rg1、Rb1均可不同程度增加α分泌酶中ADAM9、ADAM10基因表达，提高α分泌酶活性，且人参皂苷Rg1调节作用优于人参皂苷Rb1。
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| 关 键 词： | 人参皂苷RG1 人参皂苷Rb 淀粉样前体蛋白分泌酶 阿尔茨海默病 |
Effect of Ginsenoside Rg_1 and Rb_1 on Secretase in Metabolic Pathway of Amyloid Precursor Protein |
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| WEI Cui-bai,JIA Jian-ping,WANG Fen,et al.Effect of Ginsenoside Rg_1 and Rb_1 on Secretase in Metabolic Pathway of Amyloid Precursor Protein[J].Chinese Journal of Information on Traditional Chinese Medicine,2008,15(9):28-30. |
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| Authors: | WEI Cui-bai JIA Jian-ping WANG Fen |
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| Institution: | WEI Cui-bai,JIA Jian-ping,WANG Fen,et al (Department of Neurology,Xuanwu Hospital,Capital University of Medical Sciences,Beijing 100045,China) |
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| Abstract: | Objective To study effects of Ginsenoside Rg1,Rb1 on activity and genetic expression of alpha secretase in the progress of metabolism of APP.Methods Chinese hamster ovary (CHO) cell line transacted by mutant PS1 (ML46L) genes and wild type PS1 genes was used.The activity of alpha secretase was tested by fluorometric reaction.The expression of ADAM9 and ADAM10 was analysis through western blotting and RT-PCR.Results The activity of alpha secretase was increased significantly in Ginsenoside Rg1,Rb1 group than... |
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| Keywords: | Ginsenoside Rg1 Ginsenoside Rbl secretase of amyloid precursor protein Alzheimer＇s Disease |
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