雌激素替代治疗对雌鼠血管内皮细胞、心脏中雌激素受体含量的影响

目的：探讨雌激素替代治疗，高脂饮食对雌鼠心脏和肺血管内皮细胞中雌激素受体含量的影响。方法；用放射配体结合法定量检测雌激素受体，分别测定去势，高脂饮食及雌激素替代治疗后雌鼠心脏及血管内皮细胞中雌激素受体含量，并对去势前后血清雌激素及血脂水平进行检测。结果：去势及高脂饮食后心脏及血管内皮细胞中雌激素受体含量均下降（P＜0．01），但以去势为明显，雌激素替代治疗9周后雌激素受体含量明显改善；去势组雌鼠血清雌二醇水平较假手术组明显下降（P＜0．01），胆固醇水平显著升高（P＜0．01）。结论：（1）雌激素水平明显影响雌鼠心脏和血管内皮细胞中雌激素受体的表达，高脂饮食可降低雌激素受体的含量。（2）雌激素替代治疗可改善心脏和血管内皮细胞中雌激素受体含量。

The effects of ERT on the estrogen receptor content of heart and vascular endothelial cells in female rats

Objective To investigate the effects of estrogen replacement therapy (ERT) and cholesterol enriched diet on the change of estrogen receptor content of vascular endothelial cells (VEC) and hearts in female rats. Methods The receptor binding assay (RBA) was adopted to measure the estrogen receptor content, and the serum levels of estradiol and lipids were measured as well. Results The content of estrogen receptor (ER) decreased in hearts and VEC of ovariectomized and high cholesterol diet rats ( P <0.01), but more apparently in the former group. After giving ERT for nine weeks, the content of ER was elevated. Serum level of estradiol dropped and serum cholesterol level increased significantly after the female rats were ovariectomized ( P <0.01). Conclusions The expression of ER in VEC and hearts are affected by serum estrogen level, and cholesterol enriched diet decreases the content of ER. ERT may improve the content of ER of heart and vascular endothelial cells.