| Expressions of myc and ras gene in human hepatocellular carcinoma by applying the double hybridization in situ |
| |
| 引用本文： | 刘彦君,丛文铭,张秀忠,谢天培,沈锋,郭亚军,陈汉,吴孟超. Expressions of myc and ras gene in human hepatocellular carcinoma by applying the double hybridization in situ[J]. 中国人民解放军军医大学学报, 1996, 0(2) |
| |
| 作者姓名： | 刘彦君 丛文铭 张秀忠 谢天培 沈锋 郭亚军 陈汉 吴孟超 |
| |
| 作者单位： | Tumor Immunology and Gene Therapy Center,Institute of Hepatobiliary Surgery,Changhai Hospital,Second Military Medical University,Shanghai,200433 |
| |
| 摘 要： | ＥｘｐｒｅｓｓｉｏｎｓｏｆｍｙｃａｎｄｒａｓｇｅｎｅｉｎｈｕｍａｎｈｅｐａｔｏｃｅｌｌｕｌａｒｃａｒｃｉｎｏｍａｂｙａｐｐｌｙｉｎｇｔｈｅｄｏｕｂｌｅｈｙｂｒｉｄｉｚａｔｉｏｎｉｎｓｉｔｕＬｉｕＹａｎｊｕｎ（刘彦君）;ＣｏｎｇＷｅｎｍｉｎｇ（丛文铭）...
|
Expressions of myc and ras gene in human hepatocellular carcinoma by applying the double hybridization in situ |
| |
| Liu Yanjun, Cong Wenming, Zhang Xiuzhong, Xie Tianpei, Shen Feng, Guo Yajun, Cheng Han, Wu Mengchao. Expressions of myc and ras gene in human hepatocellular carcinoma by applying the double hybridization in situ[J]. Journal of Medical Colleges of PLA(China), 1996, 0(2) |
| |
| Authors: | Liu Yanjun Cong Wenming Zhang Xiuzhong Xie Tianpei Shen Feng Guo Yajun Cheng Han Wu Mengchao |
| |
| Abstract: | Objective: To establish a method of non-isotope double in situ hybridization in order to detect the expression of two kinds of oncogenes at single cell level simultaneously, and confirm the hypothesis of "model of stepwise carcinogenesis". Methods: The method of non-isotope double in situ hybridization was established with the digoxigenin (Dig) and biotin(Bio) labelled probes. The expression of two members of oncogenes of the myc and/or ras gene families (myc and N-ras, myc and K-ras, myc and H-ras, N-ras and K-ras , N-ras and H-ras, K-ras and H-ras) was further studied with the method in 10 cases of Chinese Hepatocellular Carcinomas (HCC). Results : Co-expression of two kinds of oncogenes was detected only in a few cases, reflected by coshowing two different hybridization signals ,i. e. , co-showing of Dig-myc and Bio-H-ras, Dig-myc and Bio-N-ras, Dig-myc and Bio-K-ras was observed in 2 cases, 2 cases and one case, respectively. The common characteristics were that positive cells of myc distributed diffusely, while positive cells of ras diatributed sporadically or locally among the positive cells of myc, and only a few cells exhibited co-showing of two oncogenes at single cell level. There were only 2 cases representing co-showing of two oncogenes in ras gene family (Dig-N-ras and bio-H-ras, Dig-N-ras and Bio-K-ras , respectively). The two kinds of positive cells of different ras gene represented mixed local and sporadical distribution, and co-showing of two signals was found in a few cells at single cell level. Conclusion: There are multiple oncogenes involving in tumorigenesis by their ordered activation. The activation of the ras gene family plays a role in promotion, while the activation of myc is an important event in late stage of tumorigenesis |
| |
| Keywords: | double hybridization in situ liver neoplasms oncogene ras gene myc gene |
| 本文献已被 CNKI 等数据库收录！ |
|
