| HPLC法测定龙胆草中龙胆苦苷含量 |
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| 引用本文： | 常艳琴,王德.HPLC法测定龙胆草中龙胆苦苷含量[J].黑龙江医药,2011,24(4):533-534. |
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| 作者姓名： | 常艳琴 王德 |
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| 作者单位： | 吉林敖东集团金海发药业股份有限公司,吉林,安图,133600 |
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| 摘 要： | 方法:高效液相色谱法.Diamonsil C18色谱柱(5um 150×4.6mm);流动相为甲醇:水(25:75);检测波长为270hm;流速为1.0ml/min,柱温为20℃.结果:龙胆苦苷在0.4g/ml-4.0g/ml,浓度与峰面积积分值呈良好的线性关系,r=0.9998(n=5).结论:该方法简便、快速.
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| 关 键 词： | 高效液相色谱法 龙胆苦苷 |
Determination of Gentiopicroside in Gentianae Radix et Rhizome by HPLC |
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| Institution: | Chang Yanqin,et al Jinhaifa Pharmaceutical Co.,Ltd of Jilin Aodong Group(Antu 133600) |
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| Abstract: | Methods：An HPLC method with Diamonsil C18 column was used（5um 150×4.6mm） . Methanol-water（25：75） was used as the mobile phase;The deterction wave-length was 270 nm;The flow rate was 1.0 mL/min;The column temperature was 20℃.Results：The calibration curve was linear in the range of 0.4μg/ml-4.0μg/ml for Gentiopicroside,r=0.9998（n=5） .Conclusion：The method is simple,sensitive and accurate. |
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| Keywords: | HPLC Gentiopicroside |
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