HBV-DNA与乙型肝炎血清学标志物的相关性研究

目的探讨乙型肝炎病毒DNA（HBV-DNA）与乙肝病毒血清标志物（HBV-M）之间的相关性。方法将临床筛查的600例HBsAg阳性血清标本用ELISA法检测HBV-M,同时用荧光定量PCR法检测HBV-DNA,按不同的HBV-M模式分组进行分析。结果 HBsAg和HBeAg同时阳性的标本,检测HBV-DNA阳性率为100%,明显高于HBeAg阴性模式的阳性率,亦高于HBsAg阳性的其它模式。差异均有统计学意义（P〈0.05）。结论 HBV-DNA检测在各种模式的HBV-M阳性血清中检出率差异有统计学意义,HBeAg阳性者HBV-DNA检出阳性率最高（100%）,检测乙肝患者HBV-M的同时检测HBV-DNA,对于早期诊断、判断患者传染性程度、疗效观察和预后判断具有重要的临床意义。

Study on Correlation Between DNA and Serological Markers of Hepatitis B Virus

Objective To explore the correlation between hepatitis B virus DNA(HBV-DNA) and hepatitis B virus serological markers(HBV-M).Methods Six hundred HBsAg-positive serum samples from clinical screening were tested for HBV-M by ELISA,and the samples were also detected for HBV-DNA by fluorescence quantitative PCR.Then the results were divided into different groups of HBV-M patterns for analysis.Results The positive rate of HBV-DNA in HBsAg-positive and HBeAg-positive samples was 100%,which was significantly higher than the positive rate of HBeAg-negative mode and was also higher than the rates of other HBsAg-positive modes.The differences were statistically significant(P<0.05).Conclusions The detection rate of HBV-DNA testing in a variety of patterns of HBV-M-positive sera had statistically significant difference.The rate in HBeAg-positive patients was the highest(100%).Simultaneous detection of HBV-DNA and HBV-M had an important clinical significance for early diagnosis,infectivity judgment,clinical observation and prognosis judgment.