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| 荧光光谱法研究忍冬苷与牛血清白蛋白的相互作用 | |
| 引用本文： | 谢玉荣,杜迎翔,汪豪. 荧光光谱法研究忍冬苷与牛血清白蛋白的相互作用[J]. 海峡药学, 2011, 23(7): 57-60 |
| 作者姓名： | 谢玉荣 杜迎翔 汪豪 |
| 作者单位： | 1. 中国药科大学分析化学教研室,南京,210009 2. 中国药科大学分析化学教研室,南京,210009;中国药科大学药物质量与安全预警教育部重点实验室,南京,210009;中国药科大学现代中药教育部重点实验室,南京,210009 3. 中国药科大学天然药物化学教研室,南京,210009 |
| 摘 要： | 本文首次采用荧光光谱法研究了忍冬瞢与牛血清白费白（BSA）在模拟人体生理条件下的相互作用。结果表明忍冬苷对BSA的荧光猝灭作用属于静态猝灭．且296K下结合常数Ka为3．82×10^-5L·mol^-1，结合位点数n为1．16；热力学分析表明二者主要靠氢键和范德华力结合；位点竞争实验则显示忍冬苷主要通过Site I与BSA相结合；最后还考察了几种常见的共存离子对相互作用的影响。 |
| 关 键 词： | 忍冬苷 牛血清白蛋白 荧光光谱法 |
Study on the interaction between veronicastroside and bovine serum albumin by fluorescence spectroscopy |
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| XIE Yu-rong,DU Ying-xiang,WANG Hao. Study on the interaction between veronicastroside and bovine serum albumin by fluorescence spectroscopy[J]. Strait Pharmaceutical Journal, 2011, 23(7): 57-60 | |
| Authors: | XIE Yu-rong DU Ying-xiang WANG Hao |
| Affiliation: | 1.Department of Analytical Chemistry,China Pharmaceutical University,Nanjing 210009,China;2.Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education),China Pharmaceutical University,Nanjing 210009,China;3.Key Laboratory of Modern Chinese Medicines(Ministry of Education),China Pharmaceutical University,Nanjing 210009,China;4.Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China) |
| Abstract: | The interaction between veronicastroside and bovine serum albumin were firstly studied by fluorescence spectroscopy under simulative physiological condition in this paper.The experimental data revealed that BSA fluorescence quenching initiated by veronicastroside was belong to static quenching.The binding constant Ka was 3.82×105L·mol-1 and binding site was 1.16 at 296K.Thermodynamic analysis showed that binding powers were mainly hydrogen and vander Waals force.The competitive experiments indicated that the binding of veronicastroside to BSA primarily took place in Sudlow site I.Effects of common ions on the binding constant of veronicastroside-BSA complex were also discussed in this article. |
| Keywords: | Veronicastroside Bovine serum albumin Fluorescence spectroscopy |
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